Identification and Safety of A Malolactic Bacterium RF17
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摘要: 通过形态学特征、生态学特征、生理生化特征、16SrDNA及pheS序列同源性分析对菌株RF17的种属类别进行鉴定,并通过急性毒性试验检测菌株的安全性,为菌株的开发应用提供理论基础。结果表明,RF17菌株细胞呈杆状,革兰氏反应为阳性,菌株可利用49种糖醇中的32种,经鉴定为植物乳杆菌植物亚种。该菌的MTD>61.2g·kg-1体重,根据急性毒性剂量分级属无毒,可安全应用于食品类生产。Abstract: The malolactic bacterium strain RF17was identified by its morphological,ecological,physiological and biochemical characteristics,as well as the results from the 16SrDNA and phe S sequence analyses.Its safety for food application was determined by an acute toxicity test in order to evaluate the potential for commercial development.The results showed that the rod-shaped,gram positive RF17cells had 32out of 49sugar alcohols. The strain was identified as Lactobacillus plantarum subsp.plantarum.Its bacterial MTD was greater than 61.2 g·kg-1 body weight.Based on the acute toxic dose fractionation,RF17 was considered non-toxic and safe for human consumption.
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Key words:
- strain RF17 /
- identification /
- safety /
- Lactobacillus plantarum subsp.plantarum
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