Comparison of ISSR and SRAP Markers on the Genetic Diversity Analysis of Dioscoreae
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摘要: 以90份山药种质资源为材料, 从多态性检测水平、遗传多样性检测水平、遗传距离分析和聚类分析4个方面对ISSR与SRAP揭示山药种质资源遗传多样性的差异进行分析。结果表明:SRAP标记的扩增谱带总数、平均扩增谱带数、平均多态性谱带数都远高于ISSR标记, 遗传距离范围也比ISSR标记更广, 但多态性比率差异不大;2种标记在检测遗传多样性和种群间的分化程度方面较为相似, 二者结果均表明山药种质资源总的遗传变异中约有52%变异存在于种群间, 约48%的变异存在于种群内;从聚类分析的结果上看, ISSR标记在以遗传距离为0.13时将山药资源分为4个类群, 而SRAP标记以遗传距离为0.18时将其分为5个类群。综合4个方面比较结果及SRAP标记和ISSR标记自身的特点, 认为SRAP标记更适合用于山药遗传多样性分析。Abstract: To analysis the genetic diversity of 90 germplasm, ISSR and SRAP markers were studied by polymorphisms, genetic diversity, genetic distance and cluster analysis.Our results showed that the total number of amplified bands, the average number of amplified bands and the average number of polymorphic bands obtained from SRAP marker are more than those marked by ISSR, and the range of genetic distance marked by SRAP was broader.However, there had no difference of the polymorphism ratios of the two markers.Furthermore, there had 52% variations between the populations and nearly 48% variations in populations, suggesting that the two markers are similar in genetic diversity analysis.Cluster analysis marked by ISSR showed that the dioscorea resources are classified into 4groups with a genetic distance of 0.13, and the results marked from SRAP showed that they are classified into 5 groups with a genetic distance of 0.18.In conclusion, our results suggest that SRAP marker is more suitable for the analysis on the genetic diversity of dioscoreae.
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Key words:
- Dioscorea polystachyaTurcz. /
- ISSR /
- SRAP /
- genetic diversity
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