基于全基因组的芽孢杆菌平均核苷酸同源性（ANI）分析

刘波; 胡桂萍; 唐唯其

doi:10.19303/j.issn.1008-0384.2013.09.001

基于全基因组的芽孢杆菌平均核苷酸同源性（ANI）分析

doi: 10.19303/j.issn.1008-0384.2013.09.001

福建省农业科学院农业生物资源研究所,福建福州,350003

基金项目:

国家自然科学基金项目 (2010GB2C400220)

科技部国际合作项目 (2012DFA31120)

科技部973前期项目 (2011CB111607)

农业部948项目 (2011-G25)

详细信息

作者简介:
刘波 (1957-), 男, 博士, 研究员, 研究方向:微生物生物技术 (E-mail:fzliubo@163.com)

中图分类号: Q933
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- 被引次数: 0
出版历程
- 收稿日期: 2013-07-02
- 刊出日期: 2013-09-18

Characteristic of Average Nucleotide Identity(ANI)Based on the Whole Genomes from Bacillus Species in Bacillus-like Genus

Agricultural Bio-Resources Research Institute,Fujian Academy of Agricultural Sciences

摘要

摘要: 采用Jspecies软件分析芽孢杆菌全基因组之间平均核苷酸同源性 (ANI) 的特征性。结果表明, 芽孢杆菌属间、种间及亚种间两两菌株间ANI与其分类地位相关, 具有明显属种特异性, 其中芽孢杆菌科不同属之间的ANI值分布于50%~65%;芽孢杆菌种间ANI值均低于95%, 分布范围为65%~90%, 加权平均数为70.12%, 其中90%的ANI值低于70%;芽孢杆菌亚种间ANI值分布主要范围为90%~96%, 占90%。因此, 芽胞杆菌属间的ANI鉴定标准建议定为50%~65%, 芽胞杆菌种间的ANI鉴定标准建议定为65%~90%, 芽胞杆菌亚种间的ANI鉴定标准建议定为90%~96%。同时, 基因组中的四核苷酸 (Tetra) 回归系数与ANI值具有相关性, 表现明显属种特征性, 回归拟合显示两者呈现一元二次方程关系, y=271-573.58x+399.65x2 (R2=0.981 2) , 同时高于70%的ANI值与相应的Tetra回归系数呈线性正相关, 方程式为y=178.58x-81.521 (R2=0.876 7) 。
- 芽孢杆菌 /
- 系统发育 /
- 平均核苷酸同源性 /
- Jspecies
Abstract: The characteristic of average nucleotide identity (ANI) based on the whole genomes from Bacillus species in Bacillus-like genus was analyzed using the soft of Jspecies.The results showed that there have a species specificity based on the ANI distribution and which correlate with the relationship of taxonomic and evolutionary affinities.The ANI values computed from different Bacillus-like genus were below a 65% identity or above a 50% different in orthologous genes identity.The ANI values from different Bacillus species were below 94% with a wide range from 63.77 to 93.81and the most of them ranging from 64.77% to 68.4% with the weighted average of 70.12%.The ANI value between subspecies was mainly ranged from 90% to 96%.Our results suggest that the ANI criterion of the identification for genus, species and subspecies within Bacillus are 50%-65%, 65%-90% and 90%-96%, respectively.Furthermore, Tetra regression index was correlated with the ANI value and displayed the species specificity.Regression fitting analysis showed that the equation of y=271-573.56x+399.65x2 with the correlation coefficient of 0.981 2occurred between the Tetra and the ANI values above a identity of 70 were positive correlated with Tetra regression index with the equation of y=178.58x-81.521and the correlation coefficient of 0.876 7.
- Bacillus /
- phylogeny /
- average nucleotide identity /
- Jspecies

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