Evaluation on in vitro Antioxidant Activity of Southern Wild Grape Wines
-
摘要: 以不同浓度的Vc溶液为对照, 采用分光光度法分析了‘刺葡萄’和‘桂葡1号’2种南方山葡萄酒对DPPH·和·OH的清除能力以及铁氰化钾还原力。结果表明:在一定浓度范围内, 2种山葡萄酒对DPPH·和·OH的清除能力随酒样浓度的增加而逐渐增加, 二者对DPPH·的清除能力分别是2.0mg·mL-1 VC溶液的2.75倍和3.14倍, 清除·OH的能力分别是1.0mg·mL-1 VC溶液的6.98倍和7.86倍;清除速度均为:桂葡1号葡萄酒>刺葡萄酒>VC溶液。刺葡萄酒和桂葡1号葡萄酒的铁氰化钾还原力极显著高于0.5mg·mL-1的VC溶液, 但二者差异不显著。Abstract: Using Vitamin C solution as control in different concentrations, “brier grape wine”and“Gui-pu No1 wine”were studied with UV spectrophotometric assay on their scavenging activity to DPPH·, ·OH and reducing activity to potassium ferricyanide.The results showed that scavenging activity of two grape wines increased with the wine concentration.The scavenging activity to DPPH·in two wines were 2.75and 3.14time equivalent of it in Vc (2.0 mg·mL-1) , respectively, and to·OH were 6.98and 7.86time equivalent in 1.0 mg·mL-1, respectively.The scavenging speeds to free radicals were following the order in“Gui-pu No1wine”>“brier grape wine”>VC.There was no obvious difference between two wines in reducing activities to potassium ferricyanide but were higher in both wins than in Vc (0.5mg·mL-1) .
-
[1] 魏巍, 李维新, 何志刚, 等.闽引葡萄“桂葡1号”酿造特性研究[J].福建农业学报, 2011, 26 (2) :265-268. [2] 吴莹.中国野生毛葡萄和葡萄酒抗氧化能力的研究[D].杨凌:西北农林科技大学, 2010:2-4. [3] 李志英, 张海容, 梁会艳.5种葡萄酒清除经自由基的比较[J].酿酒科技, 2006, (4) :26-28. [4] 任娜, 郭丽萍, 刘玉环, 等.国产葡萄酒清除自由基作用的研究[J].食品科学, 2005, 30 (15) :90-93. [5] ALEN-RUIZ F, GARCIA-FALCON M S, PEREZ-LAMELA M C, et al.Influence of major polyphenols on antioxidant activity in Mencia and Brancellao red wines[J].Food Chemistry, 2009, 113:53-60. [6] 蒋宝, 张振文, 张小转, 等.海拔对黄土高原地区赤霞珠果实酚类物质含量及抗氧化活性的影响[J].西北农林科技大学学报:自然科学版, 2011, 39 (6) :197-202. [7] MULERO T, PARDO F, ZAFRILLA P.Effect of principal polyphenolic components in relation to antioxidant activity in conventional and organic red wines during storage[J].Eur Food Res Technol, 2009, 229:807-812. [8] 蒋超, 陆军, 林莉莉, 等.红薯茎叶提取物抗氧化性的研究[J].中国食品学报, 2010, 10 (5) :74-77. [9] 李颖畅, 孟宪军.蓝莓叶黄酮提取物抗氧化活性的研究[J].营养学报, 2008, 3 (4) :427-429. [10] 牛广财, 朱丹, 王军, 等.沙棘酒清除自由基作用的研究[J].中国食品学报, 2010, 10 (1) :36-40. [11] 刘金串, 孟江飞, 郭志君, 等.膨大处理对红地球葡萄酚类物质及抗氧化活性的影响[J].食品科学, 2012, 33 (05) :7-12. [12] 董捷, 张红城, 李慧, 等.八种蜂花粉醇提物的体外抗氧化能力研究[J].营养学报, 2010, 32 (3) :309-312. [13] 郭志君, 房玉林, 马立娜.中国野生刺葡萄冬剪枝条多酚类物质含量及其抗氧化活性研究[J].食品科学, 2012, 33 (19) :159-163. [14] 胡博然, 阴淑贞, 闻雯, 等.干白葡萄酒清除DPPH自由基的能力及其与总酚含量的关系[J].食品与发酵工业, 2011, 37 (11) :60-65. [15] 房玉林, 孟江飞, 张昂, 等.罐储时间对赤霞珠葡萄酒中酚类化合物及抗氧化活性的影响[J].食品科学, 2011, 32 (11) :14-20.
点击查看大图
计量
- 文章访问数: 189
- HTML全文浏览量: 48
- PDF下载量: 2
- 被引次数: 0