Study of Spathiphyllum‘Vicki’Shoot Tip Culture in Vitro and Rapid Propagation
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摘要: 以白掌品种‘维克’的茎尖为外植体,对外植体表面灭菌、茎尖诱导分化、侧芽增殖与生根培养条件等进行研究。结果表明:以0.1%HgCl2表面灭菌8min为宜;适宜芽诱导的培养基为MS+BA1.5mg·L-1,萌发率高,达86%。侧芽增殖培养基选择MS+BA2.0+KT0.2+NAA0.3为佳,增殖系数为3.58。‘维克’无根苗生根培养基以2/3MS+IBA1.5+AC0.15%+蔗糖3%最适宜,生根率为95%,适宜移栽基质为泥炭土和珍珠岩比例为10∶1的混合基质。Abstract: The shoot tip of Spathiphyllum 'Vicki' was used as explant to study its conditions for rapid propagation, including disinfection time, bud proliferation and rooting culture, and so on. The result showed the best disinfection time of 0.1%HgClz was 8 rain. The best shoot induction medium was MS+BA 1.5 mg· L-1 and the induce rate was up to 86%. The best bud proliferation medium was MS+BA2.0+KT0.2+NAA0. 3, and the multiplication coefficient was 3.6. The best rooting medium was 2/3MS+IBA1.5+AC 0.15% +sucrose 3%, and the rooting rate was 95 ~. The peat soil. and pearlite matrix had a ratio of 10 : 1, which was suitable for transplanting.
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Key words:
- Spathiphyllum‘Vick’ /
- tissue culture /
- rapid propagation
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