Analysis on Genetic Structure of Parental Populations of White-plumage Mule Duck by Microsatellite Polymorphism
-
摘要: 应用8个微卫星标记对5个鸭种群进行遗传多样性检测。利用等位基因频率计算各群体的基因杂合度、多态信息含量及群体间的遗传距离,并根据遗传距离进行聚类分析。结果表明:8个微卫星标记座位平均有效等位基因数为2.888,5个鸭群的平均基因杂合度为0.592~0.658,平均多态信息含量为0.422~0.598,为中高度多态。各母本种群间的遗传距离0.187~0.445,番鸭与4个母本的遗传距离0.322~0.445。从聚类分析结果来看,中型母本与小型母本和大型母本先聚为1类,再与M18母本相聚,最后与番鸭相聚。Abstract: The genetic polymorphism of five duck breeds was detected by eight microsatellite markers.Genetic heterozygosity,polymorphic information content and genetic distance were calculated by allele frequency,and the cluster analysis was based on genetic distance.The result showed that the average effective allele number per locus was 2.888 at eight microsatellite locus,the average genetic heterozygosity was 0.592~0.658,and the average polymorphic information content was 0.422~0.598 in the five duck populations.The genetic distance was 0.187~ 0.445among the female parents,and the genetic distance was 0.322~0.445 between muscovy duck and the four female parents.The cluster analysis result showed that medium-sized female parent with mini-sized and large-sized clustered together firstly,and then clustered with M18 female parent,finally clustered with Muscovy duck.
-
Key words:
- parental of mule duck /
- microsatellite polymorphism /
- genetic structure
-
[1] 朱志明,陈晖,郑嫩珠,等.利用8个微卫星标记分析7个鸭群体的遗传多样性[J].中国畜牧杂志,2010,46(17):10-13. [2] 龚道清,张红,张军,等.运用微卫星标记分析11个鸭种(群)的亲缘关系[J].畜牧兽医学报,2005,36(12):1256-1260. [3] 李慧芳,李碧春,陈宽维,等.中国地方鸭品种资源的分子遗传多样性[J].畜牧兽医学报,2006,37(11):1107-1113. [4] 吴艳,侯水生,刘小林,等.4个微卫星标记分析6个鸭群体之间的遗传关系[J].畜牧兽医学报,2006,37(4):389-395. [5] GONZAIEZ-ESQUERRA R,LEESON S.Effects of menhadenoil and flaxseed in broiler diets on sensory quality and lipidcomposition of poultry meat[J].Br Poult Sci,2000,41(4):481-488. [6] MIEINIK M B,DAINTY R H,LUNDBY F.The effect ofevaporative air chilling and storage temperature on quality andshelf life of fresh chicken carcasses[J].Poult Sci,1999,78(7):1065-1073. [7] 李国治,连林生,鲁绍雄,等.猪血清蛋白多态性与杂种优势关系的探讨[J].中国畜牧杂志,2004,40(3):32-34. [8] VANHALA T,TUISKULA-HAAVISTO M,ELO K,et al.Evaluation of genetic variability and genetic distances betweeneight chicken lines using microsatellite markers[J].Poult Sci,1998,77(6):783. [9] BISHOP M D,KAPPES S M,KEELE J W,et al.A geneticlinkage map for cattle[J].Genetics,1994,136:619-639. [10] HEARNE C M,GHOSH S,TODD J A.Microsatellites forlinkage analysis of genetic traits[J].Trends in Genetics,1992,(8):288-294. -
点击查看大图
计量
- 文章访问数: 208
- HTML全文浏览量: 42
- PDF下载量: 3
- 被引次数: 0
下载:
