Effects of Exogenous Ethylene on Cell Wall Substances and Microstructure of Loquat Fruit
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摘要: 以“早钟6号”枇杷为原料,采用50~60mg.L-1外源乙烯胁迫处理,研究不同贮藏时间内枇杷果实硬度及细胞壁物质的变化,并对果肉组织进行显微结构分析。结果表明,外源乙烯胁迫能抑制枇杷果实硬度增加、水溶性果胶减少、粗纤维生成的幅度,对总果胶影响不大。处理组贮藏20d和30d的果肉硬度比贮藏前增加了35.9%和57.8%,而对照组则分别增加了62.5%和90.0%,贮藏40d后两组趋于接近;贮藏40d时,处理组水溶性果胶含量为贮藏前的57.4%,而对照组为48.5%,对照组粗纤维含量比处理组高0.6g.kg-1;通过果肉细胞显微结构观察,获得了细胞显微水平上的理论支持。Abstract: To study the changes on the hardness and cell wall substances of loquat fruit,50-60 mg·L-1 exogenous ethylene gas was applied to treat 'Zaozhong No.6′loquat fruit.The fruits were preserved at low temperature after treatment and the microstructure analysis of pulp tissue was conducted.The results showed that exogenous ethylene treatment repressed the fruits in developing of hardness,decreasing of water-soluble pectin and fibering synthesis,but not affected total pectin in the fruits.After 20 days and 30 days preservation,hardness of fruits in treatment group increased by 35.9% and 57.8%,respectively,in contrast,that of control group increased by 62.5% and 90.0% respectively.40 days later,fruit hardness of two groups tended to be similar to each other.On the 40th day,the water-soluble pectin of fruits in treated group was 57.4% of that before the preservation,and in control group,it was 48.5% of that without preservation.The crude fiber content of fruits was 0.6 g·kg-1 more in control group than in treatment group.The observations of microscope on pulp cell microstructure agreed with the results.
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Key words:
- exogenous ethylene /
- loquat /
- pectin /
- crude fiber /
- microstructure
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[1] 蔡冲,龚明金,李鲜,等.枇杷果实采后质地的变化与调控[J].园艺学报,2006,33(4):731-736. [2] 郑永华,李三玉,席玙芳.枇杷冷藏过程中果肉木质化与细胞壁物质变化的关系[J].植物生理学报,2000,26(4):306-310. [3] 李维新,何志刚,林晓姿.贮藏温度对“解放钟”呼吸强度的影响及MAP保鲜技术研究[J].福建农业学报,2004,19(4):250-253. [4] 潘瑞炽,王小菁,李娘辉.植物生理学[M].北京:高等教育出版社,2001:191-192. [5] ZHOU H W,SONEGO L,BEN-ARIE R,et al.Analysis ofcell wall components in juice of‘Flavortop’nectarines duringnormal ripening and woolliness development[J].Journal ofthe American Society for Horticultural Science,1999,124(4):424-429. [6] 胡花丽,梁丽松,王贵禧,等.外源乙烯对CA贮藏桃果实MDA含量、PPO和LOX活性变化的影响[J].西北林学院学报,2007,22(3):38-42. [7] 吕双双,李天来,吴志刚,等.外源乙烯对不同成熟度网纹甜瓜果实细胞壁水解酶活性的影响[J].西北农业学报,2009,18(4):344-350. [8] PALOU L,CARLOS H,DAVID G,et al.Effect of conti-nuous exposure to exogenous ethylene during cold storage onpostharvest decay development and quality attributes of fruitsand table grapes[J].Postharvest Biology and Technology,2003,27:243-254. [9] 张贺英.低温胁迫对枇杷超微结构及生理代谢的影响[D].福州:福建农林大学,2007. [10] 曹士峰.枇杷果实采后品质劣变调控及其机理研究[D].南京:南京农业大学,2008. [11] 林晓姿,何志刚,李维新,等.外源乙烯胁迫对枇杷果实若干品质及PPO活性的影响[J].福建农业学报,2009,24(5):467-470. [12] 李维新,何志刚,林晓姿.低温贮藏对早钟六号枇杷采后生理与贮藏特性的影响[J].华南热带农业大学学报,2005,11(3):1-4. [13] 尚雪波,帅鸣.柑橘皮中果胶含量的测定[J].湖南农业科学,2010,(5):88-90. [14] ANDREWS P K,LI S L.Cell wall hydrolytic enzyme activityduring development of non-climacteric sweet cherry(PrunusaviumL.)fruit[J].Journal of Horticultural Science,1995,70(4):561-567. [15] SAULNIER L,BRILLOUET J M.Structural studies of Pectinsubstances from the pulp of grape berries[J].Carbohyd Res,1988,182:63-78. [16] STEPONKUS P L,UEMURA M,WEBB M S.A constractof the cryo stability of the plasma membrane of cointer rye andspring oat-two species that widely differ in their freezingtolerance and plasma membrane lipid composition,In advancein Low-temperature[M].Berlin:Springer-Verlag,1993:2221-2312.
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