The Inhibitory Effect of Shenqinmycin on Various Developmental Stages of Phytophthora sojae
-
摘要: 为寻找防治大豆疫病的新型生物农药,离体条件下测定申嗪霉素对大豆疫霉菌不同生长发育阶段的抑制作用。结果表明,申嗪霉素对大豆疫霉菌菌丝生长、游动孢子的释放和萌发的抑制中浓度分别为2.043 6、230.370 1和0.726 5μg.mL-1,但对孢子囊形成没有抑制作用。说明申嗪霉素在离体条件下对大豆疫霉菌具有明显的抑制作用,具有防治大豆疫病的潜力。Abstract: In oder to control Phytophthora blight of soyben by new biological pesticides,the bioactivity of Shenqimycin against different stages of Phytophthora sojae was investigated in vitro.The results showed that Shenqinmycin could inhibit mycelia growth,zoospores release and germination with the effective concentration(EC50) values of 2.043 6,230.370 1 and 0.726 5 μg·mL-1 respectively,but have no effect on sporangia production.These findings suggest that Shenqinmycin can obviously inhibit the growth of P.sojae in vitro and have high potential for controlling of Phytophthora blight in soyben.
-
Key words:
- Shenqinmycin /
- Phytophthora sojae /
- Various Developmental Stages /
- Inhibition
-
[1] SUN F,KALE S D,AZURMENNDI H F,et al.StructuralBasis for Interactions of the Phytophthora sojae RxLR EffectorAvh5with Phosphatidylinositol 3-Phosphate and for Host CellEntry[J].Molecular Plant-Microbe Interactions,2012,26(3):330-344. [2] TYLER B M.Phytophthora sojae:root rot pathogen ofsoybean and model oomycete[J].Molecular Plant Pathology,2007,8(1):1-8. [3] WRATHER J A,STIENSTRA W C,KOENNING S R.Soybean disease loss estimates for United States from 1996to1998[J].Canadian Journal Plant pathology,2001,23:122-131. [4] 沈崇尧,苏彦纯.中国大豆疫病的发现及初步研究[J].植物病理学报,1991,21(4):298. [5] 代瑞平,刘海.大豆疫霉根腐病研究进展[J].大豆科技,2011,(1):20-22,26. [6] 马淑梅,丁俊杰,郑天琪,等.黑龙江省大豆疫霉根腐病生理小种鉴定结果[J].大豆科学,2005,24(4):260-264. [7] 房艳梅,左豫虎,崔素萍,等.大豆疫霉菌对甲霜灵、F500和氟吗.锰锌的敏感性及交互抗性研究[J].黑龙江八一农垦大学学报,2017,19(1):17-21. [8] 陈海霞,杜兴彬,张穗,等.申嗪霉素对水稻纹枯病的田间防效分析[J].上海农业学报,2012,28(2):69-71. [9] 张穗,何龙,许煜.申嗪霉素对小麦纹枯病菌的毒力测定及盆栽药剂[J].上海交通大学学报:农业科学版,2011,29(4):42-44. [10] 温广月,张穗,张红艳,等.新型微生物源农药申嗪霉素对水稻纹枯病菌的毒力测定及田间药效[J].植物保护,2008,34(4):143-145. [11] 郑文君,徐曙,周明国.申嗪霉素对油菜菌核病菌生物学活性的初步研究[J].农药学学报,2011,13(1):28-32. [12] GORGOLOUS S G,DEKKER J.Detection and measurementof fungicide resistance general principles[J].FAO Pl ProtBull,1982,30(2):39-71. [13] 兰成忠,陈庆河,赵健,等.诱导大豆疫霉菌大量产生游动孢子囊的最佳方法研究[J].江西农业大学学报,2007,29(4):557-560. [14] MATHERON M E,PORCHAS M.Impact of azoxystrobin,dimethomorph,fluazinam,fosety-Al,and metalaxyl ongrowth,sporulation,and zoospore cyst germination of threePhytopthoraspp.[J].Plant disease,2000,84:454-458. [15] 左豫虎,减忠蜻,刘惕若.影响大豆疫霉菌(Phytophthorasojae)游动孢子产生的条件[J].植物病理学报,2001,31(3):241-245. [16] 孔祥清,刘洋,左豫虎.氟吗啉对大豆疫霉菌的抑制作用[J].中国农学通报,2007,23(2):371-373. [17] PAPAVIZAS G C,BOWERS J H.Comparative fungitoxicityof captafol and metalaxyl to Phtophthora capsici[J].Phytopathology,1981,71(2):123-127. [18] YANG J H,SHAN J,WANG H Y.Simply enhancingthroughput of free-flow electrophoresis via organic-aqueousenvironment for purification of weak polarity solute ofphenazine-1-carboxylic acid in fermentation of Pseudomonas spM18[J].ELECTROPHORESIS,2012,33(18):2925-2930. [19] 陈治芳,王文桥,韩秀英,等.灰葡萄孢(Botrytis cinerea)对氟啶胺的敏感基线及对不同条菌剂的交互抗性[J].河北农业大学学报,2011,34(3):33-36. [20] 朱丽华.杀菌剂霜脲氰的生物素结剂的合成[J].世界农药,2002,24(5):11-14.
点击查看大图
计量
- 文章访问数: 169
- HTML全文浏览量: 44
- PDF下载量: 2
- 被引次数: 0