Genetic Diversity Analysis of Strawberry Accession with ISSR
-
摘要: 以来自不同国家的34个草莓品种为试材,利用ISSR分子标记开展分类与遗传多样性分析,建立遗传树状图,揭示品种之间的亲缘关系。结果表明:11个ISSR引物共扩增出79条DNA谱带,平均每个引物扩增7.18条,其中多态性谱带占59条,多态性比率为74.68%。供试草莓品种的遗传相似系数在0.71~0.99之间,聚类分析将34份草莓供试品种在简单相似系数0.75处分成7个类群。ISSR分子标记的分类结果与草莓的地理分布有一定的相关性;鉴别出目前福建省和广东省的主栽品种法兰地和美国品种甜查理为同物异名。Abstract: ISSR were used to detect the genetic diversity among 34 strawberry accessions from different countries,to establish genetic tree and reveal the genetic relationships between the varieties.The results showed that eleven ISSR primers produced 79 DNA bands with averaged 7.18 DNA bands each primer pair;in which 59 were polymorphic bands with the ratio of 74.68%.The genetic similarity coefficient between the varieties was 0.71 to 0.99,34 strawberry accessions were classified into 7 groups at similarity coefficient of 0.75.The results from ISSR molecular markers were correlated obviously with the geographical distribution of the Strawberry accessions.The varieties of "Fualandi" in Fujian and Guangdong province with "Sweet Charlie" in American were proved to be a synonym.
-
Keywords:
- strawberry /
- Genetic diversity /
- ISSR
-
-
赵密珍,王静,王壮伟,等.世界草莓生产和贸易[J].果农之友,2012,(6):38. 钟开勤.瓠瓜种质资源的RAPD和ISSR分析[D].福州:福建农林大学,2007. 汪伟裁.苦瓜种质资源的ISSR分析[D].福州:福建农林大学,2008. 王志刚.利用分子标记进行草莓品种鉴定的研究[D].沈阳:沈阳农业大学,2005. 谢运海,夏德安,姜静,等.利用正交设计优化水曲柳ISSR-PCR反应体系[J].分子植物育种,2005,(3):445-450. 刘万勃,宋明,刘富中,等.RAPD和ISSR标记对甜瓜种质遗传多样性的研究[J].农业生物技术学报,2002,(3):231-236. 王志刚,张志宏.分子标记在草莓遗传研究中的应用[J].辽宁农业科学,2005,(3):36-38. 陈琼娥,钟凤林,潘东明,等.草莓分子标记技术研究进展[J].亚热带农业研究,2011,(1):64-67. SARENT D J.Agenetic linkage map of microsatellite,genespecific and morphological markers in diplo Frogaria[J].Theor Appl Gente,2004,109:1385-1391.
王江波.福建荔枝种质资源的ISSR分析[D].福州:福建农林大学,2009. 朱海生,李永平,林珲,等.草莓品种的亲缘关系及其不同继代次数的叶片组培苗的RAPD分析[J].核农学报,2010,24(2):264-268. 张运涛,冯志广,李天忠.草莓品种亲缘关系的AFLP分子标记分析[J].园艺学报,2006,33(6):1199-1202. 张颖君.草莓属(Fragaria)植物亲缘关系的RAPD研究[D].保定:河北农业大学,2003. 何淑娟.利用AFLP分子标记分析草莓的遗传多样性[D].保定:河北农业大学,2007. 王志刚,张志宏,李贺.利用RAPD和SCAR标记鉴定草莓品种[J].园艺学报,2007,34(3):591-596. LANDRY B S,LI R Q,KHANNIZADEH S.A cladisticapproach and RAPD markers to characterize 75strawberrycultivars and breeding lines[J].Res Reports,1997,16:28-33.
高山,许端祥,林碧英,等.38份瓠瓜种质资源遗传多样性的ISSR分析[J].植物遗传资源学报,2007,8(4):396-400. SAGAR S P,SIRSHA M,ASHOK P G,et al.Geneticdiversity analysis of mango cultivars using inter simplesequence repeat markers[J].Current Science,2007,93(8):1135-1152.
陈朝文.丝瓜种质资源遗传多样性的ISSR分析[D].福州:福建农林大学,2009. ISSHIKI S,IWATA N,KHAN M R.ISSR variations ineggplant(MelongenaL.)and related Solanum species[J].SciHort,2008,117:186-190.
余贤美,艾呈祥.杧果野生居群遗传多样性ISSR分析[J].果树学报,2007,24(3):329-333. PREVOST A,WILKINSON M J.A new system ofcomparing PCR primers applied to ISSR fingerprinting ofpotato cultivars[J].Theoretical and Applied Genetics,1999,98(1):107-112.
FANG D Q,ROOSE M L,KRUEGER R R,et al.Fingerprinting trifoiate orange germplasm accessions withisozymes,RFLPs,and inter simple sequence repeat markers[J].Theoretical and Applied Genetics,1997,95:211-219.
TALHINHAS P,NEVES M J,LEITAO J.Inter andintraspecific genetic diversity in lupinus evaluated with AFLP,ISSR and RAPD markers[C]//Wild and cultivated lupinsfrom the tropics to thepoles:Proceedings of the 10thInternational lupin conference,2002:19-24.
LANHAM P G,KORYCINSKA A B,RENNAN R M.Genetic diversity within a secondary gene pool for RibesnigrumL.revealed by RAPD and ISSR markers[J].J Hort SciBiotch,2000,75(4):371-375.
计量
- 文章访问数: 234
- HTML全文浏览量: 48
- PDF下载量: 3
下载:
