Components of Protein Subunit in Correlation with Plant Organs of Fuhua 5 at Pod-setting Stage
-
摘要: 为深入研究荚果期花生各主要器官蛋白质亚基组分分布特点,采用SDS-PAGE电泳法,分析珍珠豆型花生福花5号荚果发育过程,植株主要器官各类蛋白质亚基及其相对浓度分布和相关性。结果表明:在荚果期,不同时期花生主要器官蛋白质谱带数差异较大,各主要器官蛋白质谱带数为籽仁>叶片>茎部=果针>根部>果壳;各器官蛋白质组分不同,分子量在32~238Ku,34Ku谱带表达的频率最大,在各主要器官都有表达,不同器官的蛋白质组分有特异性。不同器官总蛋白质亚基相对浓度差异较大,开花40~90d各器官总蛋白质平均相对浓度以籽仁最大,茎部最小。福花5号各器官总蛋白质亚基平均相对浓度排序籽仁>果针>叶片>根部>果壳>茎部。不同器官、不同分子量条带的蛋白质亚基相对浓度差异较大。根、茎、叶、果针、果壳和籽仁的蛋白质亚基相对浓度分别分布在0.01~1.84、0.01~1.47、0.02~1.57、0.01~1.94、0.02~1.43和0.01~4.59。福花5号的根、叶、仁的蛋白质中,除仁80Ku和仁的33Ku亚基与其他亚基无相关外,其他亚基间均有相关,亚基相关类型有正负相关、只有正相关、只有负相关和只有单个相关。Abstract: In order to understand the distribution of protein in plant organs of Fuhua 5 during the pod development,the protein content and component of subunits were analyzed with SDS-PAGE electrophoresis.The results showed that,the number of protein subunits in major organs was differing with the pod development and the number of protein subunits in various organ was following the order of nutleafstem=needlerootshell.The protein components were different among organs with the molecular weight from 32 to 238 Ku;in which,the subunit of 34Ku was displayed in most frequency of various organs.The relative concentrations of total protein subunits in various organs were different.The average relative concentration of nuts was the largest and it of the stem was the smallest.The average relative concentrations of total protein subunits in various organ of Fuhua5 was following the order of nut﹥needle﹥leaf﹥root﹥shell﹥stem.The average relative concentrations of protein subunits were differing between the organs and with different molecular weight.The distribution of relative concentration of protein subunits for root,stem,leaf,fruit,nut and needle was in 0.01-1.84,0.01-1.47,0.02-1.57,0.01-1.94,0.02-1.43 and 0.01-4.59,respectively.In all of protein of two peanut genotypes of root,leaf and nut,except nut80Ku and nut33Ku of Fuhua5,the other subunits were significantly related.Correlation between types of subunits had positive and negative correlation,only positive correlation,only negative significant correlation and single correlation.
-
Key words:
- peanut /
- subunit /
- correlation /
- pod-setting stage
点击查看大图
计量
- 文章访问数: 194
- HTML全文浏览量: 30
- PDF下载量: 2
- 被引次数: 0