BSA-ISCOMs对大黄鱼免疫机能及抗病力的影响

陈秀霞; 黄殿盛; 柯翎; 许斌福; 方冬兰; 龚晖

doi:10.19303/j.issn.1008-0384.2013.03.002

BSA-ISCOMs对大黄鱼免疫机能及抗病力的影响

doi: 10.19303/j.issn.1008-0384.2013.03.002

福建省农业科学院生物技术研究所,福建福州,350003

基金项目:

福建省海洋与渔业厅重点项目([2010]2-13)

福建省科技平台建设项目(2009N2003)

福建省科技计划项目——省属公益类科研院所基本科研专项(2011R1021-7、2009R10035-5)

详细信息

作者简介:
陈秀霞(1977-),女,副研究员,主要从事鱼类免疫制剂研究(E-mail:xiuxiachen@163.com);黄殿盛(1986-),男,硕士,主要从事鱼类免疫学研究。黄殿盛与陈秀霞对本文内有同等贡献;龚晖(1971-),男,研究员,主要从事鱼类病原学与免疫学研究(E-mail:ghxfjm@163.com)

中图分类号: S948
计量
- 文章访问数: 231
- HTML全文浏览量: 43
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2012-12-27
- 刊出日期: 2013-03-18

Effects of BSA-ISCOMs on Immunity Response and Disease Resistance of Pseudosciaena crocea

Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

摘要

摘要: 利用免疫刺激复合物(ISCOMs)非特异免疫增强作用和可经黏膜呈递的特点,将BSA制备成ISCOMs(BSA-ISCOMs),大黄鱼连续口服免疫10d。免疫后第15d和30d采血,测血清部分非特异性免疫指标。结果显示,免疫后第15d,试验组血清SOD、补体C3、总蛋白含量显著高于对照组(P<0.05),γ-干扰素高于对照组,但差异不显著(P>0.05),溶菌酶则显著低于对照组(P<0.05);免疫后第30d,除溶菌酶外,试验组各指标与对照组差异不显著(P>0.05)。免疫完成后第30d,创伤弧菌(Vibrio vulnificus)攻毒结果显示,对照组死亡率100%,试验组死亡率26.7%,相对免疫保护率为73.3%。因此,口服BSA-ISCOMs在一定药效期限内能有效提高大黄鱼的免疫机能,增强抗病能力。
- 牛血清白蛋白(BSA) /
- 免疫刺激复合物(ISCOMs) /
- 大黄鱼 /
- 非特异性免疫
Abstract: Immune stimulating complex(ISCOMs) is an efficient mucosal delivery system which can induce non-specific immune response.Bovine serum albumin(BSA) was incorporated into ISCOMs(BSA-ISCOMs).Pseudosciaena crocea took diets containing BSA-ISCOMs for 10 d,while the control group took diets containing PBS.At the 15th and 30th day after oral completion,serum was collected for non-specific immune parameters analysis.The results showed that at 15th day after oral completion,SOD,C3 and total protein of the fish feeding BSA-ISCOMs were significantly higher than those of the control fish(P<0.05),and interferon γ of the former was higher than that of the latter,but not significantly(P>0.05),while lysozyme of the former was significantly lower than that of the latter(P<0.05).At 30th day after oral completion,these parameters except lysozyme of the experimental group were not significantly different from the control group(P>0.05).The results of the challenge with Vibrio vulnificus at 30th day after oral completion showed that the cumulative mortality of the control and the experimental group was 100% and 26.7%,respectively,and the relative percent survival(RPS) was 73.3%.So this study indicated that BSA-ISCOMs can induce the immune response and improve the disease resistance of Pseudosciaena crocea.
- bovine serum albumin(BSA) /
- immune stimulating complex(ISCOMs) /
- Pseudosciaena crocea /
- non-specific immunity

HTML全文

参考文献(29)

[1]	谢书秋,刘振勇.闽东大黄鱼养殖现状分析与发展对策[J].福建水产,2006,(3):95-97.
[2]	ROBERTSEN B,RORSTAD G,ENGSTAD R,et al.Enhancement of non-specific disease resistance in AtlanticSalmon,Salmo salar L.,by aglucan from Saccharomycescerevisiae cell walls[J].Journal of Fish Diseases,1990,13:391-400.
[3]	吴志鹏,王三英.三联疫苗对大黄鱼常见细菌性疾病免疫保护的实验研究[J].厦门大学学报:自然科学版,2004,43(1):115-118.
[4]	许斌福,林能锋,俞伏松,等.大黄鱼溶藻弧菌外膜蛋白ISCOMs的免疫效果初步研究[J].福建农业学报,2008,23(3):244-246.
[5]	沈锦玉,许文军,尹文林,等.哈维氏弧菌灭活疫苗在养殖大黄鱼中的应用与评价[J].大连海洋大学学报,2010,25(3):210-213.
[6]	鄢庆枇,苏永全,王军,等.口服免疫添加剂对养殖大黄鱼免疫机能影响的初步研究[J].集美大学学报,2001,6(2):134-137.
[7]	杨文鸽,黄晓春,李花霞,等.葡聚糖与其羧甲基衍生物对养殖大黄鱼非特异免疫作用[J].浙江农业学报,2006,18(1):16-20.
[8]	徐后国.几种新型免疫增强剂对大黄鱼幼鱼生长、存活、免疫力及抗病力的影响[D].青岛:中国海洋大学,2010.
[9]	张春晓,麦康森,艾庆辉,等.饲料中添加肽聚糖对大黄鱼生长和非特异性免疫力的影响[J].水产学报,2008,32(3):411-416.
[10]	叶立林,支海兵.免疫刺激复合物(ISCOMS)研究进展[J].中国兽药杂志,2001,35(2):47-51.
[11]	董传甫,林天龙,龚晖,等.嗜水气单胞菌主要外膜蛋白对欧洲鳗鲡的免疫保护试验[J].水生生物学报,2005,29(3):285-290.
[12]	龚晖,林天龙,吴宗福,等.嗜水气单胞菌β-hemA-ISCOMs对鳗鲡的口服免疫效果[J].福建农业学报,2006,21(4):325-329.
[13]	田丁,许斌福,林能锋,等.创伤弧菌外膜蛋白免疫刺激复合物对欧洲鳗鲡的免疫保护性分析[J].水生生物学报,2010,34(2):431-435.
[14]	MOREIN B,EKSTRM J,LVGREN K.Increasedimmunogenicity of a non-amphipathic protein(BSA)afterinclusion into iscoms[J].Journal of Immunological Methods,1990,128(2):177-181.
[15]	方勤美,林天龙,龚晖,等.嗜水气单胞菌β-hemA重组菌表达产物ISCOMs的研制[J].福建农业学报,2004,19(4):238-242.
[16]	BRADFORD M M.A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding[J].Analytical Biochemistry,1976,72(1-2):248-254.
[17]	AMEND D F.Potency testing of fish vaccines[J].Developments in Biological Standardization,1981,49:447-454.
[18]	魏俊发,俞贤达,金道森.锰超氧化物歧化酶及其化学模拟研究[J].化学进展,1997,9(1):14-21.
[19]	CHIU S H,TSAI R T,HSU J P,et al.Dietary sodiumalginate administration to enhance the non-specific immuneresponses,and disease resistance of the juvenile grouperEpinephelus fuscoguttatus[J].Aquaculture,2008,227:66-72.
[20]	李凌,吴灶和.鱼类体液免疫研究进展[J].海洋科学,2001,25(11):20-22.
[21]	王志平,张士璀,王光锋.鱼类补体系统成分及补体特异性和功能的研究进展[J].水生生物学报,2008,32(5):760-769.
[22]	SAKAI M.Current research status of fish immunostimulants[J].Aquaculture,1999,172:63-92.
[23]	戴伟,许梓荣.鱼类干扰素系统的研究进展[J].水产科学,2008,27(5):266-270.
[24]	GRINDE B,LIE,POPPE T,et al.Species and individualvariation in lysozyme activity in fish of interest in aquaculture[J].Aquaculture,1988,68:299-304.
[25]	ACKERMAN P A,IWAMA G K,THORNTON J C.Physiological and immunological effects of adjuvantedAeromonas salmonicida vaccines on juvenile rainbow trout[J].Journal of Aquatic Animal Health,2000,12:157-164.
[26]	FLETCHER G L,HOBBS R S,EVANS R P,et al.Lysozyme transgenic Atlantic salmon(Salmo salar L.)[J].Aquaculture Research,2011,42:427-440.
[27]	FEVOLDEN S E,RED K H,GJERDE B.Geneticcomponents of post-stress cortisol and lysozyme activity inAtlantic salmon;correlations to disease resistance[J].Fish&Shellfish Immunology,1994,4:507-519.
[28]	LUND T,GJEDREM T,BENTSEN H B,et al.Geneticvariation in immune parameters and associations to survival inAtlantic salmon[J].Journal of Fish Biology,1995,46:748-758.
[29]	RED K H,FEVOLDEN S E,FJALESTAD K T.Diseaseresistance and immune characteristics in rainbow trout(Oncorhynchus mykiss)selected for lysozyme activity[J].Aquaculture,2002,209:91-101.