Optimization for Regeneration System of Embryonic Tips of Vegetable Soybean
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摘要: 以福建省菜用大豆新品种闽豆5号的胚尖为材料,研究次氯酸钠消毒方法及不同浓度潮霉素B培养基对不定芽的诱导和生根的影响。结果表明:以10%的次氯酸钠消毒10min为宜;芽伸长阶段潮霉素B筛选压力为7mg.L-1;生根培养基不添加6-BA效果更佳,即1/2 MS盐+1/2B5有机+3%蔗糖+0.56%琼脂+1.0mg.L-1 IBA,pH 5.8。Abstract: Embryonic tips from 'Mindou No.5' were used in testing the effect of NaClO on sterilization and homomycin B on screening stress for inducing of adventitious buds and root growing.The result showed that the optimal effect on sterilization was 10% NaClO for 10 min;the screening stress of hygromycin B was 7 mg·L-1 at elongation stage of buds from embryonic tips.Comparing culture mediums,the rooting culture medium presented better in root growing without 6-BA,the medium consisting of 1/2 MS salt+1/2 B5 organic matter +3% sucrose+0.56% agar+1.0 mg·L-1 IBA,pH 5.8.
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Key words:
- vegetable soybean /
- embryonic tips /
- regeneration /
- homomycin B
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[1] 张慧君,敖雪,王海英,等.菜用大豆与普通大豆产量及品质的比较[J].大豆科学,2009,28(6):1011-1015. [2] 张秋英,杨文月,李艳华,等.中国菜用大豆研究现状、生产中的问题及展望[J].大豆科学,2007,26(6):950-954. [3] 韩天富,盖钧镒.世界菜用大豆生产、贸易和研究的进展[J].大豆科学,2002,21(4):278-284. [4] 余永亮,梁慧珍,王树峰,等.中国转基因大豆的研究进展及其产业化[J].大豆科学,2010,29(1):143-150. [5] SIMMONDS D H,DONALDSON P A.Genotype screening forproliferative embryogenesis and biolistic transformation ofshort-season soybean genotypes[J].Plant Cell Reports,2000,19(5):485-490. [6] 刘海坤,卫志明.利用根癌农杆菌介导转化大豆成熟种子胚尖获得转基因植株[J].植物生理与分子生物学学报,2004,30(6):631-636. [7] 宋雯雯,刘宝辉,杨明亮,等.农杆菌介导法将SPS基因导入大豆的研究[J].大豆科学,2008,27(3):387-390. [8] 朱红林,沙爱华,符秀梅,等.转录调控基因GmLEC1转化大豆及转化方法的比较[J].大豆科学,2010,29(1):7-12. [9] 钟灿,肖深根,朱保葛,等.菜用大豆高效胚尖离体再生基因型筛选[J].大豆科学,2012,31(1):9-12. [10] 邹莉,文艺,张匀华,等.大豆子叶节对潮霉素敏感性研究[J].大豆科学,2008,27(2):267-269. [11] 刘海坤,卫志明.一种大豆成熟种子的消毒方法[J].植物生理学通讯,2002,38(3):260-261. [12] BAILEY M A,BOERMA H R,PARROTT W A.Genotypeeffects on proliferative embryogenesis and plant regeneration ofsoybean[J].In Vitro Cellular&Developmental Biology-Plant,1993,29(3):102-108. -
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