Genetic Relationship of Dioscorea Polystachya Turcz.Resources Based on ISSR Markers
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摘要: 采用ISSR标记技术,分析了94份山药种质资源的遗传多样性及亲缘关系。从100条引物中优选出24条引物,共扩增出352条清晰条带,其中多态性带344条,多态性百分率为97.7%。聚类分析结果将94份山药资源分为4类,第Ⅰ类为薯蓣群、第Ⅱ类参薯群、第Ⅲ类为山薯群、第Ⅳ类褐苞薯蓣群,各种类群的遗传距离依次为:0.053 8~0.088 4,0.035 3~0.068 2,0.053 3~0.138 2,0.054 2~0.126 6,说明4个类群内各资源间亲缘关系由远到近的顺序为:参薯>山薯>薯蓣>褐苞薯蓣。以遗传相似性为标准,以种群为单位,参薯群与山薯群亲缘关系最近;与薯蓣群亲缘关系次之;与褐苞薯蓣群亲缘关系最远。Abstract: The genetic diversity and phylogenetic relationship of 94 yam germplasm was analyzed by ISSR.24 primers were selected from 100 primers and 352 clear strips were amplified.344 clear strips were polymorphic bands and the percentage of polymorphic bands was 97.7%.Genetic cluster analysis indiated that 94 yam germplasm were divided into 4 groups and the genetic distances were Dioscorea(0.053 8-0.088 4),Dioscorea alata(0.035 3-0.068 2),Dioscorea fordii(0.053 3-0.138 2) and Dioscorea persimilis(0.054 2-0.126 6).Based on the genetic similarity and the population,results suggested that Dioscorea alata group had the closest phylogenetic relationship with Dioscorea fordii group,closer with Dioscorea group and farther with Dioscorea persimilis group.
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Key words:
- Dioscorea polystachya Turcz. /
- germplasm /
- genetic distance /
- ISSR
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