饲用羽毛肽粉的组分分离及分析

姚清华; 黄薇; 林虬; 宋永康; 林香信; 胡兵

doi:10.19303/j.issn.1008-0384.2013.01.007

饲用羽毛肽粉的组分分离及分析

doi: 10.19303/j.issn.1008-0384.2013.01.007

姚清华^1,2,
黄薇^1,2,
林虬^1,2,
宋永康^1,2,
林香信^1,2,
胡兵³

1. 福建省精密仪器农业测试重点实验室,福建福州 350003;
2. 福建省农业科学院中心实验室,福建福州 350003;
3. 福建天马饲料有限公司,福建福清,350300

基金项目:

福建省农科院科技下乡“双百”行动项目(sbmb1239)

福建省自然科学基金项目(2010J01110)

详细信息

作者简介:
姚清华(1985-),男,助理研究员,硕士,主要从事水产动物饲料营养研究(E-mail:yaoyaoshuimu@163.com);林虬(1963-),男,副研究员,主要从事从事水产动物饲料营养研究(E-mail:linqiu3163@yahoo.com.cn)

中图分类号: S816
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- 被引次数: 0
出版历程
- 收稿日期: 2012-10-15
- 刊出日期: 2013-01-18

Fractionation and Analysis of Feather Peptide for Feed

1. Central Laboratory,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350300,China;
2. Fujian Key Laboratory of Precision Measurement of for Agriculture,Fuzhou,Fujian 350003,China;
3. Fujian Tianma Feed Co.,Ltd.,Fuqing,Fujian 350300,China

摘要

摘要: 旨在研究饲用羽毛肽粉中肽分子量分布及各组分的氨基酸组成。利用AKTA purifier 100蛋白层析系统分离饲用羽毛肽粉溶液各组分。通过高分辨率Sephacryl S-100预装柱从样品中分离获得5种主要组分,利用氨基酸色谱分析各组分的氨基酸组成。通过回归方程计算出5种组分的平均分子量分别为:大于2 000 000、6 456.54、758.58、398.11、186.21。游离氨基酸组分5为天冬氨酸、苏氨酸、丝氨酸、谷氨酸;组分4为由天冬氨酸、苏氨酸、丝氨酸、甘氨酸、丙氨酸组成的二肽或三肽;组分3为天冬氨酸、苏氨酸、丝氨酸、谷氨酸、甘氨酸、丙氨酸组成的六肽或七肽;组分2的肽链长度与抗菌肽相近,氨基酸组成与组分1相同,较羽毛原毛少了胱氨酸、甲硫氨酸。结果提示:饲用羽毛肽粉富含小分子肽段,在饲料蛋白源领域具有潜在的应用价值。
- 羽毛 /
- 饲料 /
- 肽 /
- 凝胶排阻 /
- 氨基酸
Abstract: The aim of this study was fractionate and analysis of feather peptide for feed.5 components contented in feather peptide meal were separated by AKTA purifier 100 with Sephacryl S-100.The molecular weight of them were as followed: >2 000 000,6 456.54,758.58,398.11,186.21.Resultsof fractionation showed the fifth component were Asp,Thr,Ser,Glu.The fourth component were dipeptide or tripeptide,which content Asp,Thr,Ser,Gly,Ala.Amino acid composition of the third component,six peptide or seven peptide,were as followed: Asp,Thr,Ser,Glu,Gly,Ala.Peptide length of component 2,whose amino acid composition is the same as component 1,was close to antibacterial peptide.All these showed feather peptide meal was one kind of potential protein source for feed as it was rich in small peptides.
- feather /
- feed /
- peptide /
- gel exclusion /
- amino acid

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参考文献(20)

[1]	KRISHNANKUTTY N.Plant proteins in fish feed:anadditional analysis[J].Current Science,2005,89(6):934-936.
[2]	孙君社,刘清培.羽毛蛋白粉高效利用研究[J].农业工程学报,2001,17(3):125-129.
[3]	林东康,聂芙蓉,崔朝霞,等.膨化羽毛粉营养价值的评定[J].河南畜牧兽医,2001,22(9):4-5.
[4]	LATSHAW J D.Quality of feather meal as affected by featherprocessing condition[J].Poultry Science,1990(69):953-958.
[5]	裴敏雅,张洋,靳伟刚,等.复合酶降解羽毛粉的效果研究[J].福州大学学报:自然科学版,2007,35(1):142-146.
[6]	孙伟丽,耿业业,刘晗璐,等.蓝狐对不同蛋白质来源日粮干物质和粗蛋白质表观消化率的比较研究[J].动物营养学报,2009,21(6):953-959.
[7]	KIM J M,LIM W J,SUH H J,et al.Feather-degradingBacillus species from poultry waste.Process Biochemistry,2001,37(3):287-291.
[8]	沈银书,霍启光.应用回归方法估测羽毛粉氨基酸的初步研究[J].动物营养学报,2000,12(1):16-20.
[9]	崔艳红,李培庆,阮坤祥,等.羽毛粉酶法水解工艺条件优化的研究[J].贵州农业科学,2007,35(3):25-27.
[10]	王梦芝,詹爱军,高杨,等.利用实时定量PCR研究不同蛋白质饲料对嗜淀粉瘤胃杆菌生长参数的影响[J].动物营养学报,2010,22(2):327-334.
[11]	姚清华,宋永康,林虬,等.饲用羽毛肽粉的制备[J].中国饲料,2010(12):37-40.
[12]	罗钦,陈人弼,宋永康.发酵鱼粉中寡肽检测技术研究[J].福建农业学报,2008,23(3):327-330.
[13]	宋青春,齐遵利.水产动物营养与配合饲料学[M].中国农业大学出版社,北京,2010:29.
[14]	HARA H,FUNABIKI R.Portal absorption of smallpeptides in ratusunder unrestrained conditions[J].Journal ofNutrition,1984(114):1122-1129.
[15]	NEWEY H.Smyth D H.Intracellular hydrolysis ofdipeptides during intestinal absorption[J].American Journalof Physiology,1960(152):367.
[16]	宋永康,林虬,陈人弼,等.寡肽在水产饲料中的应用[J].福建农业学报,2005,20(S):63-66.
[17]	王碧莲,徐加悦,钱雪桥.小肽制品对欧鳗生长特征的影响[J].深水渔业,2011,31(2):42-43.
[18]	GLAHN R P,CAMPEN D R,VAN CAMPEN D.R.Ironuptake is enhanced in Caco-2cell monolayers by cysteine andreduced cysteinyl glycine[J].Journal of Nutrition,1997,127(4):642-647.
[19]	SPEARS J W,SEHOENHERR W D.Efficacy of ironmethionine as a source of iron for nursing pigs[J].Journal ofAnimal Science,1992,70:243.
[20]	韩文瑜,孙长江.抗菌肽的研究现状与展望[J].中国兽药杂志,2009,43(10):11-19.