Comparison of Biological Characteristics of Two Different Disease-type Duck Reovirus(MDRV and NDRV)
-
摘要: 通过电镜观察、血清交叉中和试验、病毒的细胞病变特征、病毒基因组特征及S3蛋白基因分析等方法,比较2株不同疾病型鸭源呼肠孤病毒的生物学及基因组特性差异。染毒细胞的超薄切片观察结果表明,2种病毒为球形,直径约70nm,新型鸭呼肠孤病毒(NDRV)在胞浆中呈晶格状分布,而番鸭呼肠孤病毒(MDRV)呈零星、散在分布;MDRV和NDRV之间的相关系数R值很小,抗原相关性较低;NDRV的细胞病变类型以巨融合为主,而MDRV则以细胞圆缩、坏死为主;经尿囊腔接种,NDRV能致死鸡胚,而MDRV不能致死鸡胚。MDRV及NDRV的S基因片段的迁移率明显不同;两者S3基因处在不同的进化分支。结果初步表明不同疾病型的水禽呼肠孤病毒MDRV和NDRV的生物学特性有较大差异。Abstract: Muscovy duck reovirus(MDRV) and Novel duck reovirus(NDRV),which belong to Orthoreovirus,are important pathogens of waterfowl.This study was conducted to compare the biological characteristics of MDRV and NDRV.Several methods were undertaken such as ultrathin sections observation,cross virus-neutralization test,the pathogenicity to avian embryos and cell lines,comparison of genome segment profiles and the characteristics analysis of S3 gene.The results of ultrathin sections observation showed that MDRV and NDRV viral particles were spherical and the diameter was both about 70 nm.MDRV scattered in MDEF cytoplasm,whereas NDRV particles arranged in crystal lattice.Relatedness(R) values showed that the antigen of the two viruses were generally different.MDRV′s CPE feature was cell rounding,while which of NDRV was cell fusion.NDRV induced the death of 9-day-old chicken embryos,whereas MDRV did not.The migration profiles of NDRV and MDRV genome segments were different.The S3 genes of the two viruses were at different branches of molecular phylogenetic tree.In conclusion,the biological characteristics of MDRV and NDRV were obviously different.
-
Key words:
- Novel duck reovirus /
- Muscovy duck reovirus /
- biological characteristics
-
[1] 胡奇林,陈少莺,林锋强,等.番鸭呼肠孤病毒的鉴定[J].病毒学报,2004,20(3):242-248. [2] 陈少莺,陈仕龙,林锋强,等.一种新的鸭病(暂名鸭出血性坏死性肝炎)病原学研究初报[J].中国农学通报,2009,25(16):28-31. [3] 陈少莺,陈仕龙,林锋强,等.新型鸭呼肠孤病毒的分离与鉴定[J].病毒学报,2012,28(3):224-230. [4] 陈宗艳,朱英奇,王世传,等.一株新型鸭源呼肠孤病毒(TH11株)的分离与鉴定[J].中国动物传染病学报,2012,20(1):10-15. [5] 殷震,刘景华.动物病毒学[M].第2版.北京:科学出版社,1997:223-224. [6] 殷震,刘景华.动物病毒学[M].第2版.北京:科学出版社,1997:328-330. [7] 秦卓明,徐怀英,欧阳文军,等.新城疫不同毒株交叉鸡胚中和指数及其与F和HN基因变异的相关性[J].微生物学报,48(2):226-233. [8] 梁宏伟,王长忠,李忠,等,聚丙烯酰胺凝胶快速、高效银染方法的建立[J].遗传,2008,30(10):1379-1382. [9] LAEMMLI U K.Cleavage of Structural Protein during theAssembly of the Head of Bacteriophage T4[J].Nature,227:680-685. [10] 刘红,吴志新,郁宏伟,等.一种新病毒性鸭病的初步研究[J].养禽与禽病防治,2007,(6):2-5. [11] NI Y,KEMP M C.Strain-sepcific selection of genomesegments in avian reovirus conifections[J].J.Gen.Virol.,1992,73(12):3107-3113. -
点击查看大图
计量
- 文章访问数: 233
- HTML全文浏览量: 39
- PDF下载量: 4
- 被引次数: 0
下载:
