Metabonomic Heterogeneity of Extracellular Metabolites of Three Bacillus Genera
-
摘要: 采用基于气相色谱质谱联用(GC/MS)的代谢组学方法研究了芽胞杆菌3个属胞外代谢物,得到代谢物共229种,并对结果做了PCA和分层聚类分析,结果表明供试不同属的芽胞杆代谢物种类和含量有所不同,而同属的菌株代谢物相似,同属的菌株能聚成一类。其中类芽胞杆菌属和芽胞杆菌属又聚为1个亚类,而短芽胞杆菌属聚为另1个亚类。从对3个属芽胞杆菌所分析的数据中初步鉴定得到3个差异性代谢物。Abstract: Based on metabonomics,229 extracellular metabolites from three Bacillus genera were identified by GC/MS.PCA and hierarchical cluster analysis showed that the metabonomics differed among the genera,but were similar within same genus.The strains in a genus could be clustered into one group.The strains of Paenibacillus and Bacillus clustered into one subgroup,and the stains of Brevibacillus clustered into another subgroup.In this study,three metabolic markers were identified for the three Bacillus genera.
-
Key words:
- Bacillus genera /
- metabonomics /
- extracellular metabolites /
- heterogeneity /
-
[1] 刘国红,林营志,林乃铨,等.芽胞杆菌分类研究进展[J].福建农业学报,2012,26(5):911-917. [2] 许国旺,杨军.代谢组学及其研究进展[J].色谱,2003,21(4):316-320. [3] 许国旺,路鑫,杨胜利.代谢组学研究进展[J].中国医学科学院学报,2007,29(6):701-711. [4] 邱德友,黄璐琦.代谢组学研究—功能基因组学研究的重要组成部分[J].分子植物育种,2004,2(2):165-177. [5] 郭成栓,欧阳蒲月,谢和.枯草芽胞杆菌E20发酵产生挥发性风味成分的GC/MS分析[J].中国酿造,2010,(9):153-155. [6] 刘玮玮,季静,王超,等.枯草芽胞杆菌挥发产物的杀线虫活性评价及成分鉴定[J].植物病理学报,2009,(3):304-309. [7] 陈峥,刘波,车建美,等.龙眼微生物保鲜剂挥发性物质分析[J].中国农学通报,2011,27(20):115-118. [8] 龚春燕,张道敬,魏鸿刚,等.多粘类芽胞杆菌HY96-2发酵液化学成分研究[J].天然产物研究与开发,2009,21(3):379-381,387. [9] 谢晶,葛绍荣,陶勇,等.多粘类芽胞杆菌BS04拮抗成分分离纯化及其特性[J].化学研究与应用,2004,16(6):775-777. [10] 王芳,纪明山,谷祖敏,等.枯草芽胞杆菌B36抑菌活性成分的初步提取[J].湖北农业科学,2009,(4):874-876. [11] 江木兰,赵瑞,王国平.油菜内生枯草芽胞杆菌BY-2抗油菜菌核病菌有效成分的鉴定和分离提纯[J].中国油料作物学报,2006,28(4):453-456. [12] 陈峥,刘波,车建美,等.龙眼微生物保鲜菌FJAT-0809-GLX发酵液丙酮萃取物的成分分析[J].中国农学通报,2012,27(3):294-298. [13] 陈峥,刘波,朱育菁,等.pH条件对短短芽胞杆菌FJAT-0809-GLX次生代谢物产生的影响[J].福建农业学报,2012,27(1):71-76. [14] 宋亚军,杨瑞馥,郭兆彪,等.若干需氧芽胞杆菌芽胞脂肪酸成分分析[J].微生物通报,2001,28(1):23-28. [15] BUNDY J G,WILLEY T L,CASTEL R S,et al.Discrimination of pathogenic clinical isolates and laboratorystrains of Bacillus cereus by NMR-based metabolomic profiling[J].FEMS Microbiol Lett,2005,242(1):127-136. [16] 顾渝娟,吴振先.代谢组学在植物研究中的应用[J].广东农业科学,2012,39(4):105-107. [17] 刘波.芽胞杆菌文献研究[M].广州:广东旅游出版社,2006. [18] 车建美,付萍,刘波,等.保鲜功能微生物FJAT-0809-GLX对龙眼保鲜特性的研究[J].热带作物学报,2010,31(9):1632-1640. [19] CHANGYAN YANG,BO ZHANG,JOHANNES MOEN,et al.Fractionation and characterization of bio-oil frommicrowave-assisted pyrolysis of corn stover[J].Int J Agric&Biol Eng,2010,3(3):1-8. [20] J LIU,Y LEI,F WANG,et al.Immunostimulatory activitiesof specific bacterial secondary metabolite of Anoxybacillusflavithermus strain SX-4on carp,Cyprinus carpio[J].Journalof Applied Microbiology,2011,110(4):1056-1064.
点击查看大图
计量
- 文章访问数: 142
- HTML全文浏览量: 36
- PDF下载量: 4
- 被引次数: 0