• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析

车建美 马桂美 刘波 郑雪芳 唐建阳

车建美, 马桂美, 刘波, 郑雪芳, 唐建阳. 青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析[J]. 福建农业学报, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017
引用本文: 车建美, 马桂美, 刘波, 郑雪芳, 唐建阳. 青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析[J]. 福建农业学报, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017
CHE Jian-mei, MA Gui-mei, LIU Bo, ZHENG Xue-fang, TANG Jian-yang. Identification and Analysis of Tn5 Transposed Insert Sites in Avirulent Mutants of Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017
Citation: CHE Jian-mei, MA Gui-mei, LIU Bo, ZHENG Xue-fang, TANG Jian-yang. Identification and Analysis of Tn5 Transposed Insert Sites in Avirulent Mutants of Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017

青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析

doi: 10.19303/j.issn.1008-0384.2012.11.017
基金项目: 

国家自然科学基金项目(30871667)

“十二五”农村领域国家科技计划课题(2012AA101504)

国家“948”计划项目(2011-G25)

福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y03)

国家星火科技计划项目(S20110410006)

国家“973”计划前期项目(2011CB111607)

详细信息
    作者简介:

    车建美(1977-),女,副研究员,博士,研究方向:生物技术(E-mail:chum2002@163.com);刘波(1957-),男,博士,研究员,研究方向:微生物生物技术与农业生物药物(E-mail:liubofaas@163.com)

  • 中图分类号: S432.4

Identification and Analysis of Tn5 Transposed Insert Sites in Avirulent Mutants of Ralstonia solanacearum

  • 摘要: 利用TN5插入强致病力青枯雷尔氏菌FJAT-91,获得突变株FJAT-t582和FJAT-t583。菌落形态观察及弱化指数测定结果表明,突变株FJAT-t582和FJAT-t583表现为典型的无致病力菌落形态,且弱化指数均在0.8以上。利用卡那霉素抗性基因特异性引物从突变菌株FJAT-t582和FJAT-t583中均扩增出片段大小为681bp的条带,而野生型菌株FJAT-91未扩增出任何条带,说明TN5转座子已插入其基因组中。对这2株无致病力突变株进行反向PCR测序和序列比对,分析鉴定出插入位点在编码putative diguanylate phosphodiesterase基因和Ⅲ型效应蛋白基因中,推测这2个基因的突变致使强致病力菌株表现出无致病力特征。
  • [1] 刘波,朱育菁,林抗美,等.青枯雷尔氏菌在植株体内分布及其致病力的异质性研究[J].中国农业科学,2007,40(7): 1559-1566.
    [2] 李海涛,邹庆道,吕书文,等.番茄青枯病的研究进展[J].园艺学报,2001,28(S): 649-654.
    [3] 刘渡,林营志,朱育菁,等.生防菌对青桔雷尔氏菌的致弱特性[J].农业生物技术学报,2004,12(3): 322-329.
    [4] 郑雪芳,车建美,林营志,等.基于ITS序列和RAMS标记分析青枯雷尔氏菌的遗传多样性[J].农业生物技术学报,2008,16(4): 694-700.
    [5] 李柏青,朱育菁,张赛群,等.蜡状芽孢杆菌对番茄青枯雷尔氏菌致病性的影响[J].厦门大学学报,2007,46(4): 574-577.
    [6] MCKINNEY H H.Mosaic diseases in the Canary Islands,West Africa,and Gibraltar[J].Journal of Agricultural Research,1929,39: 557-558.
    [7] LOVREKOVICH L,FARKASG L.Induced protection against wildfire disease in tobacco leaves treated with heat-killed bacteria[J].Nature,1965,205;823-824.
    [8] 何礼远,康耀卫.利用青枯菌无毒菌株和荧光假单胞菌诱导花生产生抗病性[J].植勃保护学报,1990,17(2): 113-116.
    [9] 程本亮,车建美,刘波.青枯雷尔氏菌TN5转座子无致病力突变株构建及其生物学特性[J1.农业生物技术学报,2011,19(1): 26-38.
    [10] cARMElLLE A,PRIOR P,KODJA H,et a1.Evaluation of resistance to race 3,biovar 2 of Ralstonia sokznacearum in tomato germplasm[J].Journal of Phytopathology,2006,154(8): 398-402.
    [11] STEFANI E,GIOSUE S,MAZZUCCHI U.Detection of latent infections of Ralstonia solanacearum biovar 2,race 3 in tomato crops[J].Journal of Plant Pathology,2005,87(3): 167-171.
    [12] 刘波,蓝江林,车建美,等.青枯雷尔氏菌脂肪酸型与致病性的关系[J].中国农业科学,2009,42(2): 511-522.
    [13] THUISTON H D.Tropical plant pathology[J]: At homabroad.Annual Review of Phytopathology,2001,39: 1-11.
    [14] 孙希芳.CORESTA青桔病共同试验分学组研究报告[J].烟草科技,2001,(11): 30-33.
    [15] 王羽.肖崇尉.番茄青桔病病菌无致病力菌株的分离和控病研究[J].西南农业大学学报,2004,26(4): 426-428.
    [16] 康耀卫,毛国璋,吕常胜,等.利用青枯菌胞外蛋白输出缺失突变体防治番茄青枯病的研究[J].植物保护学报,2005,22(3): 287-288.
    [17] FREY P,PRIOR P,MARIE C,et a1.Hrp-mutants of Pseudomonas solanacearum as potential biocontrol agents of tomato bacterial wilt[J].Applied and Environmental Microbiology,1994,60(9): 3175-3181.
    [18] TRIGALE A,TRIGALET D D.Use of avirulent mutants of Pseudomonas solanacearum for the biological control of bacterial wilt of tomato plants[J].Physiological and Molecular Plant Pathology,1990,36(1): 27-38.
    [19] GALPERIN M Y.Bacterial signal transduction network in a genomic perspective[J].Environmental Microbiology,2004,6: 552-567.
    [20] GALPERIN M Y,NIKOLsKAYA A N,KOONIN E V.Novel domains of the prokaryotic two-component signal transduction systems[J].FEMS Microbiology Letter,2001,203: 11-21.
    [21] ANDREW J S.DMITRI A,MARK G.The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains[J].Journal of Bacteriology,2005,187(14): 4774-4781.
    [22] BOMCHILN,WATN-ICK P,KOLTER R.Identification and characterization of a vibrio cholerae gene,mbaA,involved in maintenance of biofilm architecture[J].Journal of Bacteriology,2003,185: 1384-1390.
    [23] GARCIA B,LATASA C,SOLANO C,et a1.Role of the GGDEF protein family in salmonella cellulose biosynthesis and biofilm formation[J].Molecular Microbiology,2004,54: 264-277.
    [24] ALDRIDGE P,PAUL R,GOYMER P,et a1.Role of the GGDEF regulator PieD in polar development o{caulobacter crescentus[J].Molecular Microbiology,2003,47: 1695-1708.
    [25] BOLES B R,MCCARTER L.Vibrio parahaemolyticus scrABC,a novel operon affecting swarming and capsular polysaccharide regulation[J].Journal of Bacteriology,2002,184: 5946-5954.
    [26] THOMAS C,ANDERSON C R,CANALES S R,et a1.PsfR,a factor that stimulates psbAI expression in the cyanobacterium synechococcus elongatus PCC 7942[J].Microbiology,2004,150: 1031-1040.
    [27] BLOCK A,LI G Y,ZHENG Q F,et a1.Phytopathogen type III effector weaponry and their plant targets[J].Current Opinion in Plant Biology,2008,11(4): 396-403.
    [28] JAMES R A,COLLMER A.Type Ill secretion system effector proteins: double agents in bacterial disease and plant defense[J].Annual Review of Phytopathology,2004,42: 385-414.
  • 加载中
计量
  • 文章访问数:  162
  • HTML全文浏览量:  44
  • PDF下载量:  2
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-08-09
  • 刊出日期:  2012-11-18

目录

    /

    返回文章
    返回