Genetic Diversity Analysis of Main Male Sterile Lines for Indica Hybrid Rice Based on SSR Markers
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摘要: 采用SSR标记对我国杂交籼稻15个主要不育系进行遗传多样性分析。结果表明,在水稻基因组中平均分布的99个SSR位点其中71个SSR位点具有多态性,多态性位点率71.7%。71个SSR标记共检测到175个等位基因,每个SSR位点检测到2~5个,平均为2.47个等位基因,He平均为0.716,PIC平均为0.393。供试不育系在遗传距离0.693处聚为两大类群,广占63S单独聚为一类;另一大类包括其余4个光温敏核不育系和所有的三系不育系。聚类结果与系谱分析基本吻合。Abstract: Genetic diversity was analyzed among 15 main male sterile lines of indica hybrid rice from China with 99 pairs of SSR marker evenly distributed in the 12 chromosomes of rice.The results indicated that 71(71.1%) pairs of SSR marker showed polymorphism.There were 171 alleles detected with those 71 pairs of SSR marker.The number of alleles for per locus ranged 2-5 with an average of 2.47.The average of He was 0.716 and average of PIC was 0.393.The 15 main male sterile lines were divided into two groups at genetic distance 0.693.The sterile line Guangzhan 63S could be grouped alone.Another group was composed of four photo-thermo-sensitive genic male sterile lines and all main three-line male sterile lines.The clustering results based on SSR analysis was basically consistent with the pedigree of 15 main male sterile lines.
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Key words:
- India hybrid rice /
- male sterile lines /
- simple sequence repeats /
- genetic diversity
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[1] 张宏根,孔宪旺,朱正斌,等.粳稻三系亲本的性状特征与杂种优势分析[J].作物学报,2010,36(5):801-809. [2] 王胜军,陆作楣.中国杂交籼稻遗传多样性演变及其分析[J].江苏农业学报,2006,22(3):192-198. [3] 彭锁堂,王海岗,魏兴华,等.我国三系杂交稻主要不育系的微卫星标记多样性和遗传结构分析[J].中国水稻科学,2008,22(4):365-369. [4] 陆作楣.我国杂交稻育种研究的亮点及难点[J].中国水稻科学,2011,25(3):231-235. [5] 贺浩华,罗小金,朱昌兰,等.杂交稻部分不育系与恢复系的SSR分类[J].作物学报,2006,32(2):169-175. [6] 江云珠,汤圣祥,余汉勇,等.利用SSR标记对中国水稻品种进行遗传多样性评价和品种分类的研究[J].中国稻米,2010,16(4):19-24. [7] 陈新民,何中虎,史建荣,等.利用SSR标记进行优质冬小麦品种(系)的遗传多样性研究[J].作物学报,2003,29(1):13-19. [8] 聂永心,张丽,潘光堂,等.四川省常用玉米自交系SSR遗传多样性分析[J].分子植物育种,2005,3(1):43-51. [9] 薛林,张丹,徐亮,等.玉米抗粗缩病自交系种质的发掘和遗传多样性及其在育种中的应用[J].作物学报,2011,37(2):2123-2129. [10] 李英慧,刘燕,关荣霞,等.“十五”大豆创新种质和1963-1995年间育成品种的SSR遗传结构及遗传多样性分析[J].作物学报,2007,33(10):1630-1636. [11] 段艳凤,刘杰,卞春松,等.中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457. [12] 王丽鸳,姜燕华,段云裳,等.利用SSR分子标记分析茶树地方品种的遗传多样性[J].作物学报,2010,36(12):2191-2195. [13] DOYLE J J,DOYLE J L.A rapid total DNA preparationprocedure for fresh plant tissue[J].Focus,1990,12:13-15. [14] 盖红梅,任民.SSR数据处理宏程序DataTrans1.0[J].分子植物育种(网络版),2011,(9):1359-1365. [15] 唐梅,何光华,裴炎,等.中籼杂交稻遗传多样性的RAPD分析[J].西南农业学报,2002,15(3):7-9. [16] 陈亮,梁春阳,孙传清,等.AFLP和RFLP标记检测水稻亲本遗传多样性比较研究[J].中国农业科学,2002,35(6):589-595. [17] 杨友才,周清明,尹晗琪,等.烟草种质资源遗传多样性及亲缘关系的AFLP分析[J].中国农业科学,2006,39(11):2194-2199. [18] PEJIC I,AJMONE-MARSAN P,MORGANTE M,et al.Comparative analysis of genetic similarity among maize imbredlines detected by RFLPs,RAPDs,SSRs,and AFLPs[J].Theor Appl Genet,1998,(97):1248-1255. [19] 袁力行,傅骏骅,WARBURTON M,et al.利用RFLP,SSR,AFLP和RAPD标记分析玉米自交系遗传多样性的比较研究[J].遗传学报,2000,27(8):725-733. [20] BELAJ A,SATOVIC Z,CIPRIANI G,et al.Comparativestudy of the discriminating capacity of RAPD,AFLP and SSRmarkers and of their effectiveness in establishing geneticrelationships in olive[J].Theoretical and Applied Genetics,2003 107(4):736-744. [21] 许旭明.水稻籼粳亚种间杂交衍生系遗传基础的研究[D].福州:福建农林大学,2009. [22] 李云海,肖唅,张春庆,等.用微卫星DNA标记检测中国主要杂交水稻亲本的遗传差异[J].植物学报,1999,41(10):1061-1066. [23] 李云海,钱前,曾大力,等.中国主要杂交稻亲本的RAPD鉴定及遗传关系研究[J].作物学报,2000,26(2):171-176. [24] 肖小余,王玉平,张建勇,等.四川省主要杂交稻亲本的SSR多态性分析和指纹图谱的构建与应用[J].中国水稻科学,2006,20(1):1-7.
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