鹅源鸭1型甲肝病毒的分离与鉴定

陈红梅; 施少华; 程龙飞; 傅光华; 万春和; 黄瑜

doi:10.19303/j.issn.1008-0384.2012.11.005

鹅源鸭1型甲肝病毒的分离与鉴定

doi: 10.19303/j.issn.1008-0384.2012.11.005

陈红梅^1,2,
施少华^1,2,
程龙飞^1,2,
傅光华^1,2,
万春和^1,2,
黄瑜^1,2

1. 福建省农业科学院畜牧兽医研究所,福建福州,350013;
2. 福建省畜禽疫病防治工程技术研究中心,福建福州,350013

基金项目:

现代农业产业技术体系建设专项基金(CARS-43)

国家公益性行业科研专项(201003012)

福建省自然科学基金(2011J01113)

详细信息

作者简介:
陈红梅(1979-),女,硕士,助理研究员,主要从事畜禽传染病研究(E-mail:chenhmei052@126.com);黄瑜(1965-),男,博士,研究员,主要从事畜禽传染病研究(E-mail:huangyu_815@163.com)

中图分类号: S852.65
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- 被引次数: 0
出版历程
- 收稿日期: 2012-09-05
- 刊出日期: 2012-11-18

Isolation and Identification of Duck Hepatitis Virus A type 1 with Goose-origin

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350013,China;
2. Fujian Animal Disease Control Technology Development Center, Fuzhou,Fujian 350013,China

摘要

摘要: 2012年广东省某鹅场发生一种以肝脏出血为主要病变特征的传染病。从病死鹅肝脏中分离到1株病毒(命名为GD-01),该病毒能在96h内致死10日龄番鸭胚。人工感染8日龄雏番鸭后,濒死前出现角弓反张,病变为肝脏出血。应用鸭1型甲肝病毒特异性引物对GD-01进行RT-PCR检测,可扩增到约199bp目的条带。经RT-PCR鉴定和遗传进化分析证实该病毒分离株为鹅源鸭1型甲肝病毒。
- 鹅源 /
- 鸭1型甲肝病毒 /
- 分离与鉴定
Abstract: A disease with gosling liver bleeding as the main pathological syndrome occurred in Guangdong Province in 2012.A virus strain named GD-01 was isolated from a dead gosling,which could cause death on the duck embryo within 96 hours.8 days-old Muscovy ducklings infected with GD-01 showed the opisthotonos and liver bleeding before deuth.GD-01 was characterized by RT-PCR using specific primers for duck hepatitis virus A type 1,and an 199 bp fragment was amplified.Genetic analysis showed that the isolated strain belonged to duck hepatitis virus A type 1.
- goose-origin /
- Duck Hepatitis Virus A type 1 /
- isolation and identification

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参考文献(15)

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