Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain
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摘要: 采集福建省某猪场爆发的疑似病毒性腹泻的病死仔猪病料,利用ST细胞从病死仔猪空肠及肠系膜淋巴结材料中分离获得1株病毒,对该病毒进行快速金标检测、电镜形态学观察、中和试验及RT-PCR鉴定后,证实该分离株为TGEV,命名为TGEV福建分离株(TGEV-FJ)。该分离毒在ST细胞上的毒价约为10-6.75.(100μL)-1。TGEV-FJ株N基因全长1 149bp,编码382个氨基酸,将该基因核苷酸序列和推导的氨基酸序列与GenBank中TGEV参考毒株进行比较分析,两者核苷酸的同源性为95.4%~99.8%,氨基酸同源性为96.1%~100%。Abstract: Samples were collected from dead piglets from a swine farm suffered from severe diarrhea in Fujian,2007.To determine the causes of the disease,a virus was isolated from mestenteric lymphnodes of the piglets with ST cells.Obvious CPEs had shown that this virus could proliferate well in ST cells.TCID50,by Reed-Muench,was 10-6.0·(100 μL)-1.The virus was identified to be TGEV with neutralization test,colloidal-gold strips,electronic microscopy and TGEV N gene(1 149 bp) RT-PCR.In comparison with publicated TGEVs in GenBank,homology of nucleotide and amino acid were 95.4% to 99.8% and 96.1% to 100%,respectively.The result indicated that the isolated strain is TGEV,which was first isolated in Fujian,and named as TGEV-FJ strain.
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Key words:
- TGEV /
- isolation and identification /
- N gene /
- evolutionary analysis
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[1] 斯特劳B E,阿莱尔S D,蒙加林W L.猪病学[M].赵德明,张中秋,沈建忠,译.第8版.北京:中国农业大学出版社,2000:305-339. [2] 王劭,陈少莺,林锋强,等.猪传染性胃肠炎病毒RT-PCR检测方法的建立[J].动物医学进展,2007,28(11):12-16. [3] 丁利,陈光达,许信刚,等.猪传染性胃肠炎病毒陕西株的分离鉴定[J].中国兽医杂,2011,47(10):9-12. [4] 黄小波,曹三杰,文心田,等.猪传染性胃肠炎病毒SC-H株的分离与鉴定[J].黑龙江畜牧兽医,2008,(8):58-61. [5] 张莉,翁崇鹏,毛娅卿,等.猪传染性胃肠炎病毒河北分离株核衣壳N蛋白基因的克隆与序列分析[J].中国兽药杂志,2005,39(6):14-17. [6] 孙朝军,孙昭迪,高顺,等.仔猪病毒性腹泻的防治[J].畜牧兽医科技信息,2011,(11):85-85. [7] 李龙.2011年冬哺乳仔猪病毒性腹泻最新流行情况[J].养猪,2012,(2):96-96. [8] 刘占通,王书丽.猪细小病毒研究进展[J].上海畜牧兽医通讯,2011,(4):23-25. [9] 韩勇,李文刚,项朝荣,等.一例猪狂犬病毒分离和鉴定[J].中国动物检疫,2007,24(4):40-41. [10] 刘小萍,刘昌林,张立新,等.一起新生仔猪疑似温和型猪瘟引发腹泻的诊治[J].江西畜牧兽医杂志,2012,(1):27-28. [11] 李建强,柳纪省,胡永浩,等.猪传染性胃肠炎病毒分子生物学研究进展[J].动物医学进展,2006,27(2):1-4. [12] 宋振辉,郭万柱,韩国全,等.猪传染性胃肠炎病毒SC-Y株N基因的克隆及原核表达[J].四川农业大学报,2006,24(2):210-213. [13] KAPKE P A,BRIAN D A.Sequence analysis of the porcinetransmissible gastroenteritis coronavirus nucleocapsid proteingene[J].Virology,1986,151:41-49. -
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