DNA测序技术及其应用研究进展

刘朋虎; 林冬梅; 林占熺; 李晶

doi:10.19303/j.issn.1008-0384.2012.10.020

DNA测序技术及其应用研究进展

doi: 10.19303/j.issn.1008-0384.2012.10.020

福建农林大学国家菌草工程技术研究中心,福建福州,350002

基金项目:

国家林业局公益性行业项目(201004019)

详细信息

作者简介:
刘朋虎(1982-),男,博士,助理研究员,研究方向:食用菌遗传育种与分子生物学;林占熺(1945-),男,研究员,主要从事菌草学研究

中图分类号: Q78
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- 文章访问数: 277
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- PDF下载量: 10
- 被引次数: 0
出版历程
- 收稿日期: 2012-08-12
- 刊出日期: 2012-10-18

Advances of DNA Sequencing Technology and Its Applications

National Engineering Research Center of JUNCAO Technology,Fujian Agricultural and Forestry University, Fuzhou,Fujian 350002,China

摘要

摘要: 本文首先介绍了第一代、第二代、第三代DNA测序技术的原理、特点,在此基础上介绍了第二代测序技术在基因组测序、重测序,RNA测序,宏基因组,DNA甲基化等方面的应用。第一代测序技术以Sanger测序法为代表,操作繁琐、成本较高,不能满足大规模测序的需要。第二代测序技术以高通量、低成本为主要特点,主要包括Illumina公司的Solexa测序技术、罗氏公司的454测序技术和ABI公司的SOLiD测序技术,目前已广泛应用于生命科学研究的各个领域。第三代测序技术以单分子测序为主要特点,目前已经初见端倪,但是还没有被大规模广泛应用。
- DNA测序技术 /
- 第二代测序技术 /
- 第三代测序技术 /
- 高通量 /
- 基因组学
Abstract: In this paper,we introduced principles and characteristics of the first,second and third generation of sequencing technology,then the applications of second sequencing technology were described.Because of complicated operation and high cost,the first generation DNA sequencing technology represented by Sanger sequencing method can not meet the needs of large-scale sequencing.The second generation DNA sequencing technology characterized by high-throughout and low cost including Solexa sequencing technology of Illumina,and Applied Biosystems SOLiD and Roche 454 now has been used in many fields of life science research.The third-generation sequencing technology which can sequence single DNA molecular has also been arisen,but not been widely used in life science research
- DNA sequencing technology /
- second generation DNA sequencing technology /
- third generation sequencing technology /
- high throughout /
- genomics

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参考文献(31)

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