Effects of different current field condition on the symmetrical fusion of cotyledonary protoplasts between Cucummis sativus L. and Cucurbita ficifolia Bouche
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摘要: 从小黄瓜和黑子南瓜的幼嫩子叶分离的原生质体在不同交流电场及直流脉冲参数诱导下进行对称融合的试验结果表明,采用含0.5 mol·L-1的甘露醇溶液作为融合液,在交流场强25 V·cm-1、交流频率2 000 kHz、直流脉冲强度200 V·cm-1、幅宽40μs、脉冲次数3次的条件下,能获得最佳融合效果;融合产物经液体浅层培养再生成大量愈伤组织。该文同时对愈伤组织的遗传鉴定及植株再生进行了评述。Abstract: The studies of symmetrical fusion of protoplasts isolated from Cuixiu mini cucumber (Cucummis sativus L. ) and fig-baf gourd (Cucurbita ficifolia Bouche) were carried out. The results indicated that the conditions could lead to the best effect of cell fusion were using 0.5 mol·L-1 mannitol as fusional solution and setting the alternating current field intensity at 25 V·cm-1, the alternating current frequency at 2000 kHz, the direct current intensity at 200 V·cm-1, the direct current pulse width at 40μs and 3 direct pulse times. After cultured in shallow-layer liquid medium, large numbers of callus were regenerated. The genetic identification of callus and the plant regeneration were also evaluated.
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[1] 张兴国,刘佩英.黄瓜和南瓜原生质体融合研究[J].西南农业大学学报,1998,20(4):293-297. [2] 赖钟雄,陈振光.龙眼荔枝属间原生质体电融合[J].福建农业大学学报,2001,30(3):347-352. [3] Yamanaka H. Somatic hybrid production through protoplast fusion between Japanese radish and couliflower [J]. Hort Soc, 1990, 59 (2): 284-285. -
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