Identification of cucumber mosaic virus isolate from Dahlia pinnata
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摘要: 根据病毒生物学特性、粒体形态和血清学性质,初步鉴定在厦门地区种植的大丽花上表现花叶症状的病株上分离的1个病毒分离物属于黄瓜花叶病毒。在供试的11科39种(或品种)植物中,该分离物通过摩擦接种能侵染其中的9科35种(或品种);失活温度为50-55℃,稀释终点为10-3-10-4,体外存活期为24-28 h;病毒粒体球状,直径约28-30 nm。经间接ELISA测定,该分离物与烟草花叶病毒、南瓜花叶病毒、烟草环斑病毒、番茄不孕病毒、西番莲木质病毒的抗血清均无反应。与黄瓜花叶病毒抗血清具有强阳性反应,鉴定该病毒是黄瓜花叶病毒。Abstract: One virus isolated from Dahlia pinnata Cav. grown in Xiamen area was identified as an isolate of Cucumber mosaic cucumouirus based on its biological characteristic, particle morphology and serology of the virus isolate. The virus isolate could infect 35 species (or varieties ) of 9 families from 39 species (or varieties ) of 11 tested families. The thermal inactivation point for the virus isolate was 50-55℃; the dilution end point was 10-3 -10-4 and the longevity was 24-48 h. The electron microscopic examination showed the purified virus particle preparations was isometric particles of about 28-30nm in diameter. The virus isolate had strong positive reaction to monoclonal antibodies of CMV by enzyme-linked immunosorhbent assay, and no reaction to monoclonal antibodies of TMV, SqMV, TRSV, TAV, PFWV.
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Key words:
- Dahlia pinnata Cav. /
- Cucumber mosaic virus (CMV) /
- Identification
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