Rapid PCR detection of citrus bacterial canker disease
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摘要: 应用柑橘溃疡病菌独有的保守基因设计并合成了引物对,建立柑橘溃疡病菌PCR检测技术.PCR检测结果表明,来源于不同产地的病菌菌株均可扩增出靶标带,而水稻白叶枯病菌不能扩增出靶标带;2×10~2×105 个·ml-1的溃疡病病菌悬浮液也均可扩增出靶标带,说明该PCR技术具有很强的特异性和检测灵敏度.研究结果也表明,来源于福建、四川和安徽的柑橘溃疡病菌具有同源性.Abstract: The samples of the citrus bacterial canker disease(Xanthomonas campestris)collected from Fujian. Sichuan and Anhui in China during 2002-2003 were determined by PCR using one pair of specific primers(P1/ P2)designed based on published sequence. PCR amplification product of 493 bp target band was generated from target pathogen of X. campestris but not from non-target pathogenic bacteria of X. oryzae. Resucts showed that there was a remarkable homogeneity among the pathogens of diseased citrus from Fujian, Sichuan and Anhui, and there were specificity and sensitivity to detecting pathogen DNA of X. campestris by using PCR.
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Key words:
- Citrus bacterial canker disease /
- Xanthomonas campestris pv.citri /
- PCR /
- Detection
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[1] 刘建华,高日霞. 福建省柑橘溃疡病菌分化的初步研究[J].福建农学院学报,1987,16(3):205-213.
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