摘要:
对注射嗜水气单胞菌菌液(3106CFUmL-1)的鳗鲡,进行外周血的血相、白细胞吞噬活性、血清抗菌活力等免疫学指标的测定。结果显示:在感染的初期,感染组外周血中白细胞和淋巴细胞数量、白细胞吞噬活性及血清抗菌活力较对照组均有显著提高,其中感染组的外周血的白细胞、淋巴细胞的数量与对照组相比均有显著提高,感染组粒细胞数量在第5 d显著低于对照组(P0.05),红细胞的数量没有明显变化;感染组白细胞吞噬指数和吞噬率在注射后第1 d开始增加,第5 d和第9 d与对照组差异分别达极显著(P0.01)和显著(P0.05)水平;感染组血清抗菌活力在注射后第1 d开始上升,第9 d和第13 d感染组和对照组差异极显著(P0.01)。结果表明:在人工感染嗜水气单胞菌后,日本鳗鲡能通过白细胞主要是淋巴细胞增殖、增强白细胞吞噬活性、释放抗菌物质等方式提高对嗜水气单胞菌的免疫力。
Abstract:
Eels(Anguilla japonica) were randomly divided into the infected and control groups.Fish in the infected group were intraperitoneally injected with Aeromonas hydrophila suspension in the concentration of 3106 CFUmL-1,while those in the control group the same amount of sterile saline solution(0.85% NaCl).The eels were sacrificed to determine their nonspecific immunity on parameters including haematocyte count(i.e.,erythrocyte,leucocyte,lymphocyte and granulocyte),leucocyte phagocytic activity and serum antibacterial activity.The results showed that(a) the A.hydrophila infection increased the numbers of leucocytes and lymphocytes in the peripheral blood of A.japonica,and significantly reduced the number of granulocytes;(b) the phagocytic index and phagocytic rate increased on the 1st day after injection,and were very significantly different(P0.01) or significantly different(P0.05) on the 5th day and 9th day,as compared with the control;(c) on the 1st day after injection,the antibacterial activity of the infected fish also increased,and on the 9th day and 13th day,it was very significantly different(P0.01) from the control.The results suggested that the intraperitoneal injection enhanced A.japonicas resistance to the invading A.hydrophila by means of lymphocyte,especially,the proliferation of leucocytes,the increase in leucocyte phagocytic activity and the excretion of antibacterial substances.
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