鉴别伪狂犬病病毒强、弱毒株单克隆抗体的制备

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名

邮箱

手机号码

标题

留言内容

验证码

基金项目:

福建省科技计划项目(2002N048)

Preparation of the monoclonal antibodies discriminating virulent and attenuated strains of psudorabbies virus (PRV)

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要: 用纯化的猪伪狂犬病病毒闽A株(PRV-FA)免疫Balb/c鼠,取脾细胞和骨髓瘤细胞进行细胞融合,经间接ELISA筛选,获得11株能稳定分泌伪狂犬病毒单克隆抗体的杂交瘤细胞株,其中6株仅与强毒FA反应,而不与弱毒FB、Bartha及gE缺失疫苗株发生反应。特异性鉴定结果表明,各株单抗均不与猪瘟病毒、猪流感病毒、猪呼吸繁殖障碍综合症病毒、猪细小病毒等发生交叉反应。
- 伪狂犬病病毒 /
- 强弱毒株 /
- 单克隆抗体 /
- 制备
Abstract: The purified psudorabies virus strain FA (PRV-FA) was inoculated to Balb/c mice.Eleven monoclonal hybridoma cell lines which were able to stably excrete monoclonal antibodies against PRV were produced by means of hybridoma technique and indirect ELISA detection.The results of immunologic characteristics identification revealed that 6 hybridoma cell lines only reacted with PRV strain FA and didn’t react with PRV-FB, PRV-gE-and PRV-Bartha.And all these monoclonal antibodies didn’t react with HCV, SI, PRRSV and PPV.
- Psudorabbies virus /
- Virulent and attenuated strain /
- Monoclonal antibodies /
- Preparation

[1]	刘秀梵.单克隆抗体在农业上的应用[M].合肥:安微科学技术出版社,1994.30.
[2]	刘苏燕,董邦全.分析不同单克隆抗体识别的表位特异性的三种ELISA方法比较[J].上海免疫学杂志,1991,11(2):108.
[3]	李富强,刘尚高,董红梅,等.分泌抗猪伪狂犬病病毒(北京株)单克隆抗体杂交瘤细胞株的建立[J].中国兽医杂志,2000,26(10):8-9.

点击查看大图