柑桔黄龙病亚洲种病原的PCR-SSCP分析

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基金项目:

福建省科技厅项目(2004I019)

Analysis of citrus Huanglongbing pathogens from different places by PCR-SSCP assay

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 应用PCR-SSCP技术对采自我国柑桔主产区、泰国和法国留尼湾等8个不同地方的柑桔黄龙病亚洲种的病原(Candidatus Liberibacter astiaticus)进行研究。对引物P1、P2扩增的PCR产物进行SSCP分析,得到两条清晰且泳动速率一致的条带,表明不同地方柑桔黄龙病亚洲种病原扩增的DNA片段之间没有差异。
- 柑桔黄龙病 /
- 病原 /
- PCR-SSCP
Abstract: A polymerase chain reaction (PCR) and single-strain conformation polymorphism (SSCP) assay were applied to rapidly analyze the molecular variability in the citrus Huanglongbing pathogens (Candidatus Liberobacter asiaticus) collected from citrus orchards of different places.The result showed that there was no differences among the pathogens in PCR-SSCP by using Primer 1 and Primer 2.
- Citrus Huanglongbing /
- Pathogen /
- PCR-SSCP

[1]	田亚南,柯穗,柯冲.应用多聚酶链式反应(PCR)技术检测和定量分析柑桔黄龙病病原[J].植物病理学报,1996,26(3):243-250.
[2]	李韬,柯冲.应用Nested-PCR技术检测柑桔木虱及其寄主九里香的柑桔黄龙病带菌率[J].植物保护学报,2002,29(1):31-35.

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