摘要:
长期继代的伏令夏橙愈伤组织经扫描电镜和透射电镜观察显示,在MT基本培养基中培养20d后,细胞之间连接紧密,有少量纤丝相连.贮藏物质和细胞器丰富,细胞表面形成皱褶;50d后,细胞表面仍有山脉状皱褶.但皱褶幅度减少。细胞团表面的纤丝消失,细胞内游离核糖体增加.淀粉粒的体积减小。在胚状体诱导培养基中培养20d后,出现疣突状和纤丝结构,有的纤丝形成网络状,细胞质形成一团,没有明显的细胞器的分化,但内质网发达,游离核糖体丰富,未发现淀粉粒和胞间连丝;50d后,细胞表面的纤丝消失,细胞体积增大,彼此之问的间隙也变大,细胞器重新形成,细胞核出现,核糖体丰富并形成多聚体。
Abstract:
Scanning electron microscopic and transmission electron microscopic observation on long-term-cultured callus of Citrus sinensis cv.Valencia were carried out.The results showed that after the callus incubated on MT basic medium for 20 days, the cells were closely linked, a few fibrils appeared on the rumpled surface of the cells, abundant starch granules and organelles were observed in the cells.When cell incubated on the MT basic medium for 50 days, the surface of the cells rumpled, but the fibrils disappeared, ribosome in the cytoplasm increased but the size of starch granules reduced.The callus incubated in the somatic embryo inducing medium for 20 days led to the formation of protuberance and fibrils, sometimes the fibrils formed network, cytoplasm became a mass, and no obvious organelles, starch granules and plasmodesmata were observed) after 50 days, the fibrils on the surface of the cell disappeared, the bulk of the cells and the gap between them increased, organelles and nucleolus reformed, the ribosome were abundant and formed polyers.
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