Abstract: The genetic polymorphisms in Lycopersicon esculentum var.cerasiforme of thirty-two cultivars were investigated by using random amplified polymorphic DNA markers (RAPD).Twenty-seven primers selected from two handred primers amplified 207 DNA fragments and 139 bands showed polymorphisms that occupied 67.15% of the whole amplified bands.Each primer could amplify 2 to 12 polymorphic bands, with an average of 7.67 bands.The length of most amplified fragments ranged from 270 to 2000 bp.Cluster analysis showed that the 32 cultivars could be classified into 3 groups at the level of D 0.33, which evidently showed the variations in some important traits of Lycopersicon esculentum var.cerasiforme.