Karyotype and Ag-NORs of Anguilla australis
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摘要: 采用秋水仙素-低渗-空气干燥法、Howell(1980)的方法(银染),以肾细胞作为材料,对澳洲鳗鲡 (Anguilla australis)的核型及银染进行了研究,结果显示:澳洲鳗鲡2n=38,核型公式为2n=16m+4sm+18t,NF =58,其染色体经快速银染后表明,澳洲鳗鲡的sm2其中一条染色体短臂末端出现银染位点。Abstract: Chromosome specimen from kidney cells were prepared by using colchicines-low concentration-air drying method and Howell’s (1980) Ag-NORs staining method.The karyotype,Ag-NORs of Anguilla australis was observed.The result showed that the chromosome number of A.australis was 2n=38.The karyotype of A.australis was 2n=16m+4sm + 18t,NF = 58.The Ag-NORs staining pots could be found on the tips of short arm of sm2.
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Key words:
- Anguilla australis /
- karyotype /
- Ag-NORs
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[1] LEVAN A D.Nomenclature for centromeric position on chromosomes[J].Hereditas,1964,52:201-220. [2] HOWELL W M,BLACK D.Visualization of ribosomal gene activity:Silver stains proteins associated with rRNA transcrbed from oocyte chromosomes[J].Chromosomes,1977,62 (4):361-367. -
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