Inhibitory efficacy of the supernatant fluid femented by strain Bs-8093 Bacillus sphaericus on Ralstonia sotanacearum
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摘要: 研究发现球形芽孢杆菌Bs-8093菌株的发酵上清液对青枯雷尔氏菌强致病力菌株有抑制作用,不同配方发酵上清液对青枯雷尔氏菌强致病力菌株F.1.2-010618-07V的校正抑制率不同,最高达82.94%,最低44.93%。采用正交试验对球形芽孢杆菌Bs-8093菌株发酵培养基进行筛选,结果表明:棉籽粉、牛肉浸膏和初始pH对 Bs-8093菌株活菌数及其发酵上清液对青枯雷尔氏菌的校正抑制率都有显著影响。试验获得的最佳摇瓶配方为:棉籽粉5.0%、牛肉浸膏0.3%(质量分数),初始pH 7.4。Abstract: The present study found that the supernatant liquid of Bacillus sphaericus Bs-8093 strain had inhibiting effect on the Raistonia solanacearum virulent strain.The proofread inhibit rate variated with different prescription of Bacillus sphaericus fermented supernatant liquid to Raistonia solanacerum virulent strain F.1.2-010618-07V.the highest was up to 82.49%,the lowest was 44.93%.An orthogonal design was used to screen fermentative medium of Bacillus sphaericus isolate Bs-8093.The results showed that cottonseed powder, beef extract and initial pH had significant influence on the number of bacterium and the proofread rate for its supernatant fluid to Raistonia solanacearum.The most appropriate fermentative medium was composed of cottonseed powder 5.0%.beef extract 0.3%, initial pH 7.4.
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