Effects of nitrate nutrition on active oxygen metabolism in the leaves of wheat seedlings under heat-stress condition
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摘要: 以早衰型小麦品种L14为材料,研究高温胁迫下氮素营养与活性氧代谢的关系,结果表明:在轻度和严重高温胁迫下,氮素营养均能提高活性氧代谢相关酶(SOD、CAT)的活性,降低膜脂过氧化的伤害。但是活性氧代谢非酶组分的变化各异,其中在37℃轻度高温胁迫时,随着氮素浓度的增加,AsA含量明显升高,GSH反应不敏感;42℃高温胁迫时,氮素营养明显降低AsA和GSH的含量。说明在低于大约37℃的胁迫温度下,氮素营养才能够提高活性氧的清除能力,减少活性氧的伤害作用,从而缓解高温胁迫。Abstract: The relationship between nitrate nutrition and active oxygen metabolism in the pre-senescent wheat variety L14 under heat-stress condition was investigated.The results indicated: under the conditions of either moderate or severe heat stress, nitrogen nutrition improved the activity of enzymes(SOD, CAT) related to active oxygen metabolism, reduced the harm of ex-oxidation (MDA) of membrane lipid.However, the change of non-enzymic components (AsA, GSH) in active oxygen metabolism showed difference.The contents of AsA apparently rise but that of GSH appear insensitive with increment of nitrogen concentration during moderate heat-stress; nitrogen nutrition evidently promoted reduction of contents of AsA and GSH at 42℃heat stress.It means nitrogen nutrition can raise scavenging capacity of active oxygen, reduce its harming function and finally alleviate the high temperature stress when the stress temperature is lower than about 37℃.
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Key words:
- wheat /
- heat-stress /
- nitrate nutrition /
- active oxygen
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