大白菜下胚轴不定芽再生过程中内源激素和多胺含量的变化

朱丽华; 张彩琴; 盛小光; 朱月林

大白菜下胚轴不定芽再生过程中内源激素和多胺含量的变化

1.
南京农业大学园艺学院, 江苏, 南京, 210095;
2.
南京农业大学农学院, 江苏, 南京, 210095

基金项目:

江苏省科技厅资助项目(BC2003306);教育部留学回国人员科研启动基金([2001]498)

详细信息

通讯作者:
朱月林(1963- ),男,教授,博士生导师(E-mail:ylzhu@njau.edu.cn).

中图分类号: S634.1;Q945.31
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出版历程
- 收稿日期: 2005-06-08
- 修回日期: 2005-12-22
- 刊出日期: 2006-06-15

Changes of endogenous hormone and polyamine contents during in vitro adventitious shoot regeneration from hypocotyI explants of Chinese cabbage

1.
College of Horticulture, Nanjing Agricultural University, Nanfing, Jiangsu 210095, China;
2.
College of Agronomy, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China

摘要

摘要: 研究了大白菜[Brassica campestris ssp.pekinensis(L.)Olsson]下胚轴不定芽再生过程中内源激素和多胺含量的动态变化。结果表明,芽原基出现以前(接种后0～8d)外植体内源玉米素核苷(ZRs)和脱落酸(ABA)含量上升,而吲哚乙酸(IAA)含量下降;不定芽出现(接种后8d)后变化趋势相反;ABA/IAA值和ZRs/IAA值在培养初期(接种0～8d)增大,不定芽出现以后逐渐减小。不定芽分化过程中外植体内源腐胺(Put)、精胺(Spm)和亚精胺(Spd)含量都表现为先上升后下降,均在培养第8d时达到峰值;Spm/Put值和Spd/Put值的变化趋势也与此相似。说明大白菜下胚轴不定芽的再生与内源激素和多胺含量及平衡有密切关系。
- 大白菜 /
- 下胚轴 /
- 不定芽再生 /
- 内源激素 /
- 内源多胺
Abstract: Dynamic changes of endogenous hormone and polyamine contents were investigated during in vitro shoot regeneration from hypocotyl explants of Chinese cabbage [Brassica campestris ssp.pekinensis (L.) Olsson].The results were as follows.Before shoot primordia occurred (0 to 8 days after inoculation), endogenous zeatin ribosides (ZRs) and abseisic acid (ABA) contents increased, while indole-3-acetic acid (IAA) content decreased.After shoot promordia occurred (on the 8th day of inoculation), the contrary changing patterns of ZRs, ABA and IAA contents were observed.At the early stage of culture (0 to 8 days after inoculation), ABA/IAA and ZRs/IAA ratios increased, then gradually decreased after adventitious shoots occurred.Endogenous putreseine (Put), spermine (Spm) and spermidine (Spd) contents increased at the early stage and then gradually declined during the culture course, these indexes reached their peaks on the 8th day of culture.Similar changing patterns were also observed in the ratios of Spm/Put and Spd/Put.The results indicated that endogenous hormone and polyamine contents and their balance were closely associated with the in vitro adventitious shoots regeneration from hypocotyl explants of Chinese cabbage.
- Brassica campestris ssp.pekinensis(L.) Olsson /
- hypocotyl /
- adventitious shoot regeneration /
- endogenous hormone /
- endogenous polyamine

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参考文献(4)

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