Culture and influence factors of isolated-microspore from cauliflower (Brassica oleracear L.var.botrytis L.)
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摘要: 以80份早中熟花椰菜栽培种为试材进行游离小孢子培养,研究了影响胚胎诱导的主要因子。结果表明,有20份材料诱导出胚状体,诱导率为25%;16%蔗糖培养液能较好地保持小孢子活性;13%蔗糖是小孢子发育的最适浓度;加液培养可显著提高产胚量;取材前6d内气温在10～20℃、取盛花前期至盛花中期的花蕾、选择单核靠边期至双核期的小孢子、经33℃热激处理48h后暗静止培养的产胚量最高。Abstract: Isolated microspores of eighty early and middle cauliflower cultivars were cultured.The main factors affecting the embryo induction of the microspore were studied.The results showed that embryoids had been obtained from twenty cultivars, the inducement ratio was up to 25%.It was advantage for microspore survival in the medium with 16%sucrose.The optimal concentration of sucrose for microspore development was 13%.When adding liquid to the medium during the culture, the embryo yield could be significantly improved.The optimum donators for isolated-microspore culture were the buds selected in the prophase to medial phase of full bloom and the microspore selected at the late uninucleate stage to binueleate stage at 10—20℃within six days, after the heat-shock treatment at 33℃for 48h and continued culture in darkness, the embryo yield was the highest.
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[1] DIAS J S.Effect of asctivated charcoal on Brassica oleracea microspore culture embyogenesis[J].Euphytica,1999,108:65-69. -
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