摘要:
利用RAPD和AFLP技术对半番鸭2个羽色池DNA进行检测,探讨两种技术对半番鸭羽色性状标记的效果。结果显示:(1)RAPD方法扩增带和特异性条带都较少,37个引物共扩增到297个清晰条带(平均每个引物4条);在37个引物中,7个引物含有羽色特异性条带,共15条,其中自羽半番鸭特有的条带为10条。(2)AFLP方法荧光图谱清晰度高,扩增带丰富,16对引物组合共检测1000多条扩增带(平均每个引物组合30多);在16引物中,4个引物组合检测到羽色特异性条带,共90条,仅白羽半番鸭特有的条带可达37条。可见,采用AFLP技术对半番鸭羽色标记效果更佳。
Abstract:
The labeling effect of plumage color trait of mule duck were studied by RAPD and AFLP techniques.Theresults indicated that:①The bands were less in RAPD method, 297 clear bands were amplified by 37 primers in all.On average,4 bands were amplified from a primer.In total 37 primers,only 15 specific bands were obtained by 7 primers, thereinto 10 specific bands belonged to white-plumage mule buck.②AFLP fluorescent map was clear and bands were abundant ,more than 1000 bands were amplified by 16 primers combinations.In 16 primers combinations, 90 specific bands were obtained by 4 primers combinations, in which 37 specific bands belonged to whiteplumage mule buck.AFLP technique was more suitable to be used in studying plumage color trait in mule duck.
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