一例引起欧洲鳗鲡死亡的嗜水气单胞菌分离与鉴定

王凡

一例引起欧洲鳗鲡死亡的嗜水气单胞菌分离与鉴定

王凡

1.
福建省水产技术推广总站, 福建福州 350003

详细信息

作者简介:
王凡(1978-),女,硕士,工程师,主要从事水产养殖病害防控技术研究(E-mail: wangfanfz@yahoo.com.cn)

中图分类号: S943
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出版历程
- 收稿日期: 2011-03-18
- 修回日期: 2011-05-13
- 刊出日期: 2011-08-15

Isolation and Identification of a Pathogen from an Infected European Eel(Anguilla Anguilla)

WANG Fan

1.
Fujian Provincial Fishery Technical Extention Center, Fuzhou, Fujian 350003, China

摘要

摘要: 从患病欧洲鳗鲡内脏组织中分离获得1株菌FZ01,对该菌进行了形态学、药物敏感性试验、动物感染试验和血清型分析,并进行了VITEK全自动微生物分析系统鉴定。结果显示:该菌为革兰氏阴性短杆菌,端圆,散在,TCBS琼脂上呈细小黄色菌落。VITEK全自动微生物系统鉴定该分离株为致病性嗜水气单胞菌。动物感染试验证明,对欧鳗和小白鼠具均有一定的致病性,2107 CFU攻毒健康欧洲鳗,48h内致死率达100%;药敏试验结果表明,该菌对复方恩诺沙星、环丙沙星、四环素敏感,但对复方新诺明、阿莫西林等多种药物不敏感。血清学试验显示该菌与福建省主要血清型O∶Ah10501的菌体凝集价为1∶27,属福建省主要流行株。
- 嗜水气单胞菌 /
- 分离鉴定 /
- 欧洲鳗鲡
Abstract: The strain FZ01 was isolated from a diseased Anguilla Anguilla taken from a fish farm suffering from an outbreak of unknown pathogen.The virulence,morphological,physiological and biochemical characteristics and serotype of the FZ01 were investigated.Oxidase test,catalase test and a glucose fermentation test were done by A VITEK automatic microbiological identification analysis.The results showed that the strain FZ01 was pathogenic for both eel and mouse.The fatality rate of 2107CFU for eel was 100% in 48 h.It was confirmed as the causative agent of the epidemic.FZ01 was Gram-negative bacilli,and the results showed that it was Aeromonas hydrophila.Tests of the pathogens susceptibility to antibiotics demonstrated its sensitivity to Enrofloxacin,tetracycline,and ciprofloxacin,but resistance to amoxicillin and sulfamethoxazole.Serologic tests showed that this strain's cell agglutination titer with the Fujian Province serotype O∶Ah10501 was 1∶27.These results suggested that we should take full account of serotype features in disease control process,and select the appropriate drugs and immune agents.
- Aeromonas hydrophila /
- iso1ation and identification /
- Anguilla anguilla

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参考文献(8)

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