Proliferation of Ralstonia solanacearum marked with rifampicin on the tuber of ginger
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摘要: 利用姜青枯菌Zb01抗利福平霉素标记法,研究其在姜块表面及块茎组织中的增殖动态,结果表明:标记菌株Zb01用1×102cfu·ml-1浸种姜块6h或1×107cfu·ml-1浸种姜块0.5h,播种于盆钵1h即可从姜块表面分离到标记菌株;菌量为1×104cfu·ml-1浸种姜块6h或1×107cfu·ml-1浸种姜块0.5h,播种于盆钵48h也可从块茎组织中分离到标记菌株,从姜块表面分离获得的菌落数多于块茎组织。标记菌株在姜块表面呈先降后迅速回升再降后缓慢增加的消长动态,在块茎组织中的消长动态基本是:15d前缓慢增加,到20d后急剧上升。Abstract: Proliferation dynamics of rifampicin marked strain of Ralstonia solanacearum Zb01rif on the surface and within the tuber of gingers were investigated.The results showed that Zb01rifcould colonize on the ginger surface when the ginger was treated with Zb01rif 102cfu·ml-1for 6 h or 107cfu·ml-1for 0.5h and planted into the soil for 1h.The Zb01rif could be isolated within the tuber of ginger when the ginger was treated with Zb01rif at 10 cfu·ml-1 for 6h or 107 cfu·ml-1 for 0.5h and planted into soil for 48 h.The population of the Zb01rifon the surface of ginger was higher than that within the ginger.After the ginger was treated with Zb01rif and planted into soil, the population of Zb01 on the surface of ginger declined first, and then increased rapidly and declined slowly in the end.The population of Zb01rif within the ginger increased slowly within 15 days and it increased rapidly after 20 days.
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Key words:
- ginger /
- Ralstonia solanacearum /
- proliferation
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[1] 戢俊臣,张敏,刘铭,等.四川省姜瘟病菌生物型鉴定初报[J].四川农业大学学报,2004,22(4):391-393. -
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