Screening of antagonistic strains against Fusarium oxysporum f. sp. cubense on banana
-
摘要: 从香蕉健株根际土壤中分离到130个菌株,采用平板对峙生长法,筛选出对香蕉枯萎病菌4号生理小种有拮抗作用的菌株35个。经室内复筛试验有16个菌株对枯萎病菌抑菌带的宽度达6～11mm,拮抗系数大于2.0,其中有7个菌株拮抗作用稳定。盆栽试验结果表明:拮抗菌FB5、T2WF、W10、WDWS和FB2培养液在接种病菌液后50 d的防治效果可达81%以上;拮抗菌FB5、T2WF、T2WC和W10培养液对香蕉苗生长有一定的促进作用。Abstract: Thirty-five rhizosphere strains with inhibitory activity to Fusarium oxysporum f.sp.cubense race 4 were screened by pairing culture on PSA from 130 strains which isolated from the healthy banana rhizosphere soil.Among which 16 strains showed good antagonistic effects with 6-11mm inhibition zone diameter and>2.0 antagonistic coefficient, 7 of 16 strains showed steady antagonistic effects.The results showed that the culture solution of antagonistic strains FBS, FB2, T2WF, T2WC and W10, watered into injured banana roots, could give more than 81%control efficiency on the 50th day after inoculating the pathogen.Furthermore, the culture solution of antagonistic strains FB5, T2WF, T2WC and W10 could enhance the growth of banana seedlings.
-
Key words:
- Fusarium oxysporum f.sp. cubense /
- antagonistic strains /
- screening
-
[1] 刘景梅,王壁生,陈霞,等.广东香蕉枯萎病菌生理小种RAPD技术的建立[J].广东农业科学,2004(4):43-47. [2] 曹理想,田新莉,周世宁.香蕉内生真菌、放线菌类群分析[J].中山大学学报,2003,42(2):70-73. [3] 李长松.拮抗细菌生物防治植物土传病害的研究进展[J].生物防治通报,1992,8(4):168-172. -
点击查看大图
计量
- 文章访问数: 2264
- HTML全文浏览量: 127
- PDF下载量: 604
- 被引次数: 0
下载:
