H9亚型禽流感病毒的分离及初步鉴定

朱春华; 江斌; 刘斌琼; 林甦; 陈珍; 黄瑜

H9亚型禽流感病毒的分离及初步鉴定

1.
福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心, 福建福州 350013

基金项目:

福建省科技计划重点项目(2009N4001);福建省农业科学院青年创新基金(2009QB-15)

详细信息

作者简介:
朱春华(1980-),女,硕士,助理研究员,主要从事动物病毒学研究

通讯作者:
黄瑜(1965-),男,博士,研究员,主要从事动物传染病研究(E-mail: huangyu _ 815@163.com)

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出版历程
- 收稿日期: 2011-05-04
- 修回日期: 2011-06-03
- 刊出日期: 2011-08-15

Isolation and Preliminary Identification of H9 Subtype Influenza A Viruse

1.
Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

摘要

摘要: 2011年从福建省2个肉鸡场分离到2株病毒,分别为FZ-04株和FZ-11株。血凝试验结果表明,分离株尿囊液可凝集1%鸡红细胞。血凝抑制试验结果表明,分离株尿囊液只被抗禽流感病毒H9亚型标准阳性血清特异性抑制。采用RT-PCR法对分离株M基因和H9亚型HA基因保守片段进行扩增,分别扩增出229bp和732bp特异性目的片段。结果表明,分离株为A型H9亚型禽流感病毒。HA基因遗传进化分析结果表明,分离株与代表株CK/FJ/G9/09亲缘关系最近,属于国内常见的CK/BJ/1/94群系。
- 禽流感病毒 /
- 肉鸡 /
- 分离
Abstract: 2011 two strains(FZ-04 and FZ-11)were isolated respectively from two broiler farms in Fujian.Hemagglutination test results showed that the isolated allantoic fluid can induce 1% chicken erythrocytes agglutination.Hemagglutination inhibition test results showed that the isolates allantoic fluids were only inhibited by avian influenza virus subtype H9 standard serum specificly.Specified and conserved M(Matrix)gene and HA(Hemagglutinin)gene of H9 subtype were amplified by RT-PCR with the segment lengths of 229bp and 732bp respectively.The results indicated that the isolated strains were H9 subtype of influenza A virus.The phylogenetic analysis of HA genes showed that isolates and representative strains CK/FJ/G9/09 have a closest relationship,belonging to the major sublineage CK/BJ/1/94 in China.
- avian influenza virus /
- broiler /
- isolation

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参考文献(4)

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[2]	万春和,傅光华,程龙飞,等.A/Chicken/Zhejiang/H J/2007(H 9N2)禽流感病毒 8 基因测序及遗传进化分析[J]. 农业生物技术学报, 2009,(5):750-757.
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