白灵侧耳菌株间的RAPD分析

基金项目:

福建省自然科学基金资助项目(B0310022)

RAPD analysis on Pleurotus nebrodensis (Inzengae) QuèL. strains

1.
Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Lianjiang Seed Company, Lianjiang, Fujian 350500, China

摘要: 采用CTAB法提取白灵侧耳菌丝基因组DNA,利用RAPD技术分析13个白灵侧耳菌株间的亲缘关系。结果表明,从80个随机引物筛选出11个引物,共扩增出66条带,其中多态条带50条,多态性程度为75.76%。D=0.46时,聚类分析结果可以将供试材料分为2个类群,即子实体形态为小漏斗型、柄较长的菌株聚为一类,子实体形态为手掌或马蹄型、柄很短的菌株聚为一类。揭示了白灵侧耳菌株间的亲缘关系。
- 白灵侧耳 /
- RAPD /
- 遗传多样性 /
- 聚类分析
Abstract: Total genome DNA of P.nebrodensis mycelium was extracted by CTAB and the genetic relationships among 13 P.nebrodensis strains were analyzed by RAPD.11 primers of 80 random primers produced stable results.Among 66 bands obtained from 11 primers,50 bands were polymorphic bands which occupied 75.76% of the total amplified bands.Cluster analysis showed that the materials could be divided into 2 groups at the level of D=0.46,which evidently showed that the funnel-shaped fruitbody with a longer stem strains were grouped together,the horseshoe or palm-shaped fruitbody with a very short stem strains were grouped together.The cluster analysis of varieties revealed the relationships among P.nebrodensis strains.
- Pleurotus nebrodensis (Inzengae) QuèL. /
- RAPD /
- genetic diversity /
- cluster analysis

[1]	黄年来,吴经纶,陈忠纯,等.18种珍稀美味食用菌栽培[M].北京:中国农业出版社,1997:8-16.
[2]	陈文良,刘宇,王丽珍,等.不同配方培养料对白灵侧耳生长的影响[J].食用菌学报,2004,11(1):37-41.
[3]	唐启义,冯光明.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002.
[4]	曾东方,罗信昌.应用RAPD分析快速鉴定外生菌根蘑菇分离物的真伪[J].林业科学,2001,37(6):59-64.