SSCP polymorphism of the HSPA1A gene and its relationship with heat tolerant capacity of Jersey and Holstein cows
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摘要: 设计引物扩增HSPA1A基因的5’侧翼区,通过PCR SSCP技术分析启动子区的多态性,寻找SNPs位点,分析发现:扩增片段大小280 bp,扩增产物具有基因多态性。供试的70头娟姗牛和荷斯坦奶牛共发现4种基因型,分别为AA型、BB型、AB型和AC型,卡方检验奶牛在该位点基因频率差异极显著(P0.01),未达到Hardy Weinberg平衡。AC型奶牛耐热性能高于其他基因型(AA,AB,BB)奶牛,差异显著(P0.05),建议作为抗热应激的奶牛选育分子遗传标记。Abstract: The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5’-flanking region of the HSPA1A gene.The PCR-SSCP products of primer showed polymorphisms and could be divided into four genotypes: AA,AB,AC,CC among 70 Jersey and Holstein cows.The ac genotypic cows showed higher heat tolerant capacity than those of AA,AB,and BB genotype.These mutation sites can be used as molecular genetic markers to assist the selection for anti-heat stress cows.
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Key words:
- Jersey cow /
- holstein cow /
- HSPA1A gene /
- heat tolerant capacity
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