摘要:
采用代谢物水解与衍生化同步进行,液液萃取,串联质谱(ESI+)MRM检测,外标法定量,成功测定了鳗鱼肌肉组织中4种硝基呋喃类代谢物残留量,标准曲线线性良好,定量定为0.5ng·g-1,检测限为0.25ng·g-1,在添加浓度0.5~10ng·g-1范围内,4种代谢物添加回收率为70%~130%。方法快速、简便、经济,符合出口日本、欧盟等国家的检测限量要求(1.0ng·g-1)。
Abstract:
An external method based on liquid chromatograph-electrospray ionization-mass spectrometry was developed for the determination of low-level residues of four nitrofuran in eel muscle.The procedure entailed an acid-catalyzed release of protein-bound metabolites,followed by the derivation,liquid-liquid extraction,positive electrospray ionization applying multiple reaction and monitoring two transitions for each compound.The standard curve was reliable,the LOQ was 0.5 ng·g-1,the nitrofuran metabolite recovery rates at 5 fortification levels(i.e.,0.5,1.0,2.0,5.0,10.0 ng·g-1) were 70%-130%.The LOD was 0.25 ng·g-1.The method was rapid,simple economical and its LOQ below the minimum performance limit of 1 ng·g-1 set by Japan and the EU.
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