The use of RAPD markers in population genetic analyses of cage-cultured Pseudosciaena crocea(Richardson) from Fujian,China
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摘要: 运用DNA随机扩增多态检测(RAPD)技术和4种OPV引物,对采自福建平潭和罗源的3份养殖大黄鱼样品进行遗传多样性分析。在检出的39个位点中,14个位点显性频率为1,可作为筛选大黄鱼物种标记的参考位点。样品多态位点检出率平均48.2%。样品平均杂合度(H)分别为0.251 5、0.275 5和0.257 5。2004年采自平潭和罗源的两份样品在部分已知位点存在明显的基因型频率差异,显示网箱群体之间可以出现某种程度的遗传分化。Abstract: Genetic variation among three samples of cage-cultured,Pseudosciaena crocea from Luoyuan and Pingtan,Fujian were investigated using RPAD techniques.Four OPV random primers were employed for DNA amplification through PCR.There were 39 loci detected and their representative bands were labeled as genetic markers for population genetic analyses.Forteen loci were found to be monomorphic.They were considered potential candidates for the selection of species-specific genetic markers.On average,each sample had 48.2% polymorphic loci.Nei’s average heterozygosities (H) for respective samples were 0.2515,0.2755,and 0.2575.Fishes from Pingtan and Luoyuan collected in 2004 displayed conspicuous differences in genotype frequency at many of the observed loci,indicating that the population of caged fish could differentiate in genetic structures to a certain extent.Meanwhile,the present PCR results worked out with OPV-02 revealing two distinguishable electrophoregrams that separated samples from Luoyuan collected in 2004 and in 2005.It is proposed herein that this OPV-02 distinction might be the first evidence that demonstrates genetic divergence between the so-called geographic races of Pseudosciaena crocea along the eastern coast of China.
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Key words:
- Pseudosciaena crocea /
- RAPD /
- genetic markers /
- sample heterozygosity /
- genotype frequency /
- geographic race
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