摘要:
对猪伪狂犬病病毒闽A株(Fa株)gB、gC、gD基因进行了克隆和序列测定,结果表明:gB、gC、gD基因序列全长分别为2 745 bp、1464 bp、1 215 bp,编码914、487、404个氨基酸残基组成的多肽。序列分析结果显示:Fa株与其他毒株gB、gC、gD基因核苷酸序列的同源性为94.9%~99.9%,氨基酸序列的同源性为91.4%~99.9%。不同PRV毒株gB、gC、gD基因在核苷酸和氨基酸水平上高度保守。基于gB、gC、gD基因的进化树分析表明,中国分离毒株与韩国分离毒株可归为一个进化分支,而日本分离株与欧美分离株归为另一个分支。在前一个分支里,闽A株与鄂A株的亲缘关系特别近,3个基因的同源率均在99.5%以上。在gB蛋白氨基酸序列全长上中国分离株(Ea株、Fa株)比欧美分离株多了1个氨基酸,氨基酸残基的突变主要集中在第50~100氨基酸。在gC基因序列第186~211 bp,Ea株、Fa株比欧美分离株多插入了21个碱基。在gD蛋白氨基酸序列全长上,Ea株、Fa株比其他分离株多了2~6个氨基酸。在gD基因序列第803~837 bp,Fa株核苷酸AGGCCC串联重复最多,达到7个。
Abstract:
The gB,gC,and gD genes of Pseudorabies Virus(PRV) Fa strain were cloned and sequenced.The nucleotide sequence and its deduced amino acid sequence were analyzed using computer programs.The results revealed that the gB,gC,and gD genes were 2745 bp,1464 bp,and 1215 bp long encoding corresponding to proteins 914,487,and 404 amino acids,respectively.The sequence of nucleotides and predicated amino acids were compared with those of other published PRV strains.The results showed that gB,gC,and gD gene nucleotide sequence homology was 94.9%-99.9%,while the amino acid sequence homology was 91.4%-99.9%.The gB,gC,and gD gene shared highly conserved nucleotides among PRV strains.The molecular phylogenetic tree,based on these three genes,showed that the Chinese PRV isolates and the Korean PRV isolate could be grouped into one evolution branch,while the European and American PRV isolates and the Japanese PRV isolate could belong to another branch.In the former branch,the PRV strains Ea and Fa are so closely related to one another that the homology of these three genes were all above 99.5%.The gB proteins of the Chinese PRV isolates(Ea strain and Fa strain) had an amino acid residue more than the European and American isolates and amino acid substitutions happened primarily in the amino acid residues 50 to 100.In the 186-211 nucleotide sites of gC gene,Ea strain and Fa strain had 21 nucleotides more insertion compared to the European and American isolates.The gD proteins of Ea strain and Fa strain were 2-6 amino acids residues more than other strains.For Fa strain,the tandem repetition number of nucleotides AGGCCC in the gD nucleotides 803 to 837 added up to seven,which was the most among all PRV strains.
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