Effects of pig farm wastewater on the micro-nucleus and abnormal nucleus in erythrocytes of Misgurnus Anguillicaudatus
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摘要: 利用泥鳅红细胞微核技术检测养猪场污水的诱变活性。结果表明:泥鳅红细胞微核率及核异常率在2d后就呈现统计上的显著增加,并随培养时间的延长呈先升高后降低的趋势,在第5d达到最大值;在不同浓度污水的染毒试验中,泥鳅红细胞微核率和核异常率呈现明显的剂量依赖性增加,且微核率与核变形率和空白对照组相比达到显著或极显著差异水平,表明养猪场污水具有一定的诱变活性。Abstract: The micronucleus test with the erythrocytes of the Misgurnus Anguillicaudatus was adopted to study the mutagenic activity of wastewater from pig farms.The results showed that the micro-and anomalous nuclear rates significantly increased after 2 days of treatment,peaked on the 5th day,and declined a afterward.In another experiment where varying concentrations of the wastewater were used,the rates increased with a significant dosage-dependency.The differences between the tests and the control were very significant,indicating that pig farm wastewater was mutagenic to a certain degree.
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