摘要:
采用阿利新蓝与过碘酸雪夫氏染色方法(AB-PAS)(pH 2.6)和不同pH(1.0、2.5、3.1)的阿利新蓝染色法观察花鲈消化道(舌、食道、胃、肠)粘液细胞的类型与分布。根据AB-PAS染色结果将花鲈粘液细胞分成Ⅰ～Ⅳ4种类型:Ⅰ型为红色,AB阴性,PAS阳性,含中性粘多糖;Ⅱ型为蓝色,AB阳性,PAS阴性,含酸性粘多糖;Ⅲ型为紫红色,AB与PAS均为阳性,主要含有PAS阳性的中性粘多糖,同时含有少量AB阳性的酸性粘多糖;Ⅳ型为蓝紫色,AB与PAS均为阳性,主要含有AB阳性的酸性粘多糖,同时含有少量PAS阳性的中性粘多糖。不同pH的阿利新蓝染色结果为pH 2.5和pH 3.1溶液显示酸性粘液蓝色,pH 1.0溶液仅显示弱的和强的硫酸化酸性粘液蓝色。花鲈舌粘液细胞较少,以Ⅱ型为主;食道含有大量粘液细胞,以Ⅲ型和Ⅳ型为主;胃中只含大量Ⅰ型粘液细胞;肠中也有大量粘液细胞,以Ⅲ型和Ⅳ型为主。通过对各部位粘液细胞的分类和比较,可以看出粘液细胞的分布和类型与其所在部位执行的功能有密切的关系。
Abstract:
Types and distribution of mucous cells in the digestive tracts (i.e., tongue,esophagus,stomach,and intestines) of Lateolabrax japonicus were observed and analyzed by using AB-PAS (alcian blue and periodic acid schiffs reaction) and Alcine Blue staining at different pH (pH1.0,2.5,and 3.1).According to AB-PAS,4 types of the mucous cells in the digestive tracts of L.japonicus were observed: TypeⅠ,Ⅱ,Ⅲ and Ⅳ.TypeⅠwas pure red,AB negative and PAS positive containing neutral mucoitin.TypeⅡ was pure blue,AB positive and PAS negative containing acidic mucoitin.Type Ⅲ was purple reddish,both AB and PAS positive,PAS positive more than AB containing mainly neutral mucoitin and a few acidic mucoitin.TypeⅣ was blue purple,both AB and PAS positive,AB positive more than PAS containing mainly acidic mucoitin and a few neutral mucoitin.The Alcine Blue staining at pH2.5 and pH3.1 showed blue from the acidic mucous, and at pH1.0,either weak or strong blue from the vitriolic acidity mucous.The statistical results showed that in different parts there were different types and densities of mucous cells.Only a small quantity of mucous cells existed in tongue,mostly typeⅡ.On the other hand,large quantities of mucous cells were found in esophagus,stomach,and intestines.Most of them in esophagus and intestines were of Type Ⅲ and Type Ⅳ,and all of the mucous cells in the stomach belonged to TypeⅠ.The types and distribution of mucous cells closely related to the functions of the organs where they were found.
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