土荆芥精油对菜青虫毒杀、拒食和生长抑制作用的研究

黄玉清; 魏辉; 刘剑; 吴玮; 占志雄

土荆芥精油对菜青虫毒杀、拒食和生长抑制作用的研究

1.
福建省农业科学院植物保护研究所, 福建, 福州, 350013

基金项目:

福建省科技厅项目(2005I004);福建省自然科学基金项目(B0320003,B0410015);福建省青年科技人才创新项目(2004J010)

详细信息

通讯作者:
魏辉(1972- )男,博士,副研究员,从事植物源农药与昆虫毒理的研究.

中图分类号: S436.341.2;S482.3
计量
- 文章访问数: 2085
- HTML全文浏览量: 108
- PDF下载量: 734
- 被引次数: 0
出版历程
- 收稿日期: 2007-04-18
- 修回日期: 2007-09-01
- 刊出日期: 2007-09-15

Toxic,antifeedant and growth inhibitory effects of Chenopodium ambrosioides essential oil against Pieris rapae larvae

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 采用室内生物测定方法,研究土荆芥(Chenopodium ambrosioides L.)精油对菜青虫(Pieris rapae L.)的触杀、熏蒸、拒食和生长抑制作用。结果表明,土荆芥精油对菜青虫1～5龄幼虫的触杀毒力大小顺序依次为3龄2龄1龄4龄和5龄,对菜青虫3龄幼虫的熏蒸作用的致死中浓度(LC50)明显高于1龄幼虫,对4龄幼虫明显高于1龄和2龄幼虫,但对1龄和2龄、2龄与3龄、3龄与4龄幼虫的熏蒸效果没有明显差异;对4龄幼虫拒食作用的测定结果表明,菜青虫拒食率随着土荆芥精油浓度升高而升高,处理后48h的拒食中浓度(AFC50)明显高于处理后24 h;土荆芥精油对菜青虫4龄幼虫生长发育有明显的抑制作用,蛹重和化蛹率均随着土荆芥精油浓度升高而显著下降,蛹重比率的抑制中浓度(EC50)为200.6466μg·g-1,化蛹率的EC50为126.4382μg·g-1。
- 菜青虫 /
- 土荆芥精油 /
- 毒杀 /
- 拒食 /
- 生长抑制
Abstract: Bioassays were employed to investigate contact toxicity,fumigant toxicity,anti-feeding and growth inhibitory functions of essential oil extracted from Chenopodium ambrosioides on Pieris rapae larvae.Dropping bioassay showed that the order of contact toxicity of C.ambrosioides essential oil to P.rapae larvae was 3rd instar2nd instar1st instar4th instar and 5th instar.There was a higher fumigant activity of the essential oil to the 3rd instar larvae of P.rapae than 1st instar,and to the 4th insatr than the 1st and 2nd instar.However,no significant differences between the 1st and the 2nd,the 2nd and the 3rd,or the 3rd and the 4th instar larvae were found.The anti-feeding assay showed that increasing concentration of essential oil decreased the anti-feeding rate,and the AFC50 (i.e.,effective concentration causing 50% antifeeding rate) of 48h was significantly higher than that of 24h.The results from the developmental inhibition assay indicated that the pupae weight and pupation rates decreased with the increasing essential oil concentration.The EC50 (i.e.,effective concentration causing 50% inhibition of pupae weight or pupation rates as compared to the control) on the pupae weight and pupation rates of P.rapae were 200.6466 μg·g-1 and 126.4382 μg·g-1,respectively.
- Pieris rapae (L.) /
- essential oil of Chenopodium ambrosioides /
- toxicity /
- anti-feeding /
- developmental inhibition

HTML全文

参考文献(5)

[1]	AKHTAR Y,ISMAN M B.Comparative growth inhibitory and antifeedant effects of plant extracts and pure allelochemicals on four phytophagous insect species[J].Appl Ent,2004,128:32-38.
[2]	吴文君,刘惠霞,朱靖博,等.天然产物杀虫荆-原理、方法、实践[M].西安:陕西科学技术出版社,1998.
[3]	李兵,魏辉,黄建,等.土荆芥精油的超临界CO2莘取条件及杀虫活性的初步研究[J].华东昆虫学报,2006,15(4):305-308.
[4]	国家药典委员会编.中华人民共和国药典2005年版(一部)[M].北京:化学工业出版社,2005.
[5]	李学锋,张文吉,王成菊.三种药剂对不同龄期菜青虫敏感性测定[J].农药学学报,1999,1(1):84-86.