应用氯化锂法小量提取质粒DNA

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基金项目:

国家烟草专卖局资助项目(110200601011)

A new method—LiCl for extracting bacterium plasmid DNA

1.
College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Sanming University, Sanming, Fujian 365000, China

摘要: 用改良的氯化锂方法小量提取质粒DNA.结果表明,该方法所提取的质粒DNA的质量与常规碱裂解法获得的质粒DNA基本一致,可以满足分子生物学基础实验的要求;同时该方法简化了试验步骤,质粒DNA提取时间缩短了24 min.
- 质粒DNA /
- 氯化锂
Abstract: A new method to extract small amount of plasmid DNA with LiCl was developed.The results showed that the quality of plasmid DNA extracted by using this method was basically identical to that of the conventional alkalinelysis.The method satisfied the need for basic molecular biology experiments,and the procedure was simplified shortening the extracting time by 24 min.
- Plasmid DNA /
- LiCl

[1]	F.奥斯伯,R.布伦特,R.E.金斯顿,等.精编分子生物学实验指南[M].北京:科学出版社,1998:17-19.
[2]	姚红艳,赵双宜,夏光敏.改良尿素-氯化锂方法提取成熟小麦种子总RNA[J].中国生物工程杂志,2003,23(4):86-88.

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