稻田固氮细菌分离物的PCR-DGGE及其序列同源性分析

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基金项目:

福建省科技计划项目(2001Z026)

PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria

1.
Biotech Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
College of Life Science, Fujian Agriculture and Forest University, Fuzhou, Fujian 350002, China

摘要: 采用PCR-DGGE法对从福建稻田取得的8个固氮细菌分离物进行分子鉴定,通过测序比对,发现它们与中慢生根瘤菌(Mesorhizobium sp.)、鞘氨醇单胞菌(Sphingomonas sp.)、根瘤菌(Rhizobium sp.)、慢生根瘤菌(Bradyrhizobium sp.)、克雷伯氏杆菌(Klebsiella sp.)、伯克霍德尔氏菌(Burkholderia cepacia)、假单胞菌(Pseudomonas sp.)、诺卡氏菌(Nocardioides)和红球菌(Rhodococcus sp.)9个属的固氮细菌具有高度同源性.
- PCR-DGGE /
- 水稻土 /
- 固氮细菌
Abstract: Eight bacterial isolates,which originated from paddy fields in Fujian,were analyzed using 16SrDNA-PCR-DGGE.Through sequencing and Blast analysis,9 N2-fixing bacteria strains were believed to belong to Mesorhizobium sp.,Sphingomonas sp.,Rhizobium sp.,Bradyrhizobium sp.,Klebsiella sp.,Burkholderia cepacia,Pseudomonas sp.,Nocardioides and Rhodococcus sp.,due to their high homology with the related accessions in Genbank.
- PCR-DGGE /
- paddy soil /
- nitrogen-fixing bacteria

[1]	WATANABE K,KIDAMA Y,HARAYAMA S.Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting[J].Microbiol Methods,2001,44(3):253-262.

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