Optimization of medium formulation and conditions for growing biocontrol bacteria ZB-6 using Uniform Design
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摘要: 用均匀设计法对枯草芽孢杆菌ZB-6的发酵培养基进行了优化,最佳培养基配方为:在每升培养液中,蔗糖4.82 g、牛肉膏3.1 g、黄豆饼粉11.01 g、硝酸铵.21 g、磷酸氢二钾.04 g、磷酸二氢钾2.84 g.优化验证实验结果为6.7×109 cfu·mL-1明显高于原发酵培养基结果2.36×108 cfu·mL-1.Abstract: ZB-6,a Bacillus subtilis strain obtained from healthy tomato leaf,showed a strong inhibitory activity toward plant pathogens.The Uniform Design was used to optimize the medium formulation for ZB-6 fermentation in shaking flasks.The identified formulation was∶sugar 4.82 g,beef extract 3.15 g,bean cake powder 11.01 g,ammonium nitrate 5.21 g,dipotassium hydrogen phosphate 5.04 g,and potassium dihydrogen phosphate 2.84 g.It increased the ZB-6 biomass concentration from 2.36×108 to 6.7×109 cfu·mL-1.
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Key words:
- Bacillus subtilis /
- medium formulation optimization /
- uniform design
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[1] 刘莉,辛振祥.配方优化设计方法简介[J].橡塑技术与装备,2004,30(10):8-12. -
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